青藤碱对大鼠心脏移植慢性排斥反应抑制作用的实验研究  

Role of alkaloid sinomenine in chronic rejection in the rat heart transplantation model

作　　者：徐广全[1] 金相元[1] 杨国才[1] 张策[1] 刘大仲[1] 夏求明[1] 

机构地区：[1]哈尔滨医科大学附属第二医院胸外科,150086

出　　处：《中华心血管病杂志》2008年第2期167-170,共4页Chinese Journal of Cardiology

摘　　要：目的探讨青藤碱对大鼠心脏移植慢性排斥反应抑制作用的可能机制。方法PVG大鼠作为供体、DA大鼠作为受体，所有大鼠在移植后的10d给予环孢素A，受体大鼠被分为单用青藤碱组、单用环孢素A组和两药联合治疗组。移植物经形态学测定和免疫组织化学分析。用逆转录-聚合酶链反应评估成纤维细胞生长因子、血管内皮生长因子、内皮素-1的表达。结果与未治疗组和环孢素A组受体大鼠相比，青藤碱组大鼠心脏移植物的血管病较轻（血管分数分别为2．63±0．41、2．21±0．45、2．21±0．45）。两药联合治疗组比单用任何一种药的移植物血管病都轻（1．65±0．55）。单用青藤碱不影响bFGF、VEGF和ET-1的表达。青藤碱和环孢素A联合用药明显地减少bFGF、VEGF和ET-1的表达。单用青藤碱不能抑制抗供体抗体的形成物。结论青藤碱在慢性心脏移植物排斥模型中有治疗价值。青藤碱联合环孢素A能有效抑制心脏移植物血管病，其机制可能与抑制移植物内血管的发生、血管紧张度和组织重构的介质表达有关。Objective To evaluate the possible role of alkaloid sinomenine （SIN） on chronic rejection in rat heart transplantation model. Methods After a brief course of cyclosporine A （ CsA）, DA recipients of PVG hearts were treated with placebo, SIN, CsA, or a combination of both drugs. Grafts were analyzed morphometrically and by immuno-histochemistry. Expressions of basic fibroblast growth factor （bFGF）, vascular endothelial growth factor（VEGF）, and endothelin 1 （ET-1） were assessed by reverse transcription-polymerase chain reaction. Results Cardiac grafts of SIN-treated rats showed a mild degree of vasculopathy compared with untreated rats or CsA-treated recipients. Degree of vasculopothy was significantly reduced in rats treated with combined SIN and CsA than rats receiving either drug alone. Treatment with SIN alone did not affect gene expressions of bFGF, VEGF, and ET-1 while expressions of bFGF, VEGF, and ET-1 were significantly reduced by combined treatment with SIN and CsA. Conclusion These results demonstrated a potential value of SIN, in combination with low-dose CsA to attenuate the vasculopathy in this rat model of chronic cardiac allograft rejection.

关 键 词：心脏移植 移植物排斥 青藤碱 

分 类 号：R686[医药卫生—骨科学]