色素上皮细胞衍生因子与胶质瘤侵袭的相关性  

The inhibitory effect of pigment epithelium-derived factor on migration of gliomas

作　　者：张弢[1] 关明[1] 吕元[1] 

机构地区：[1]复旦大学附属华山医院检验科,上海200040

出　　处：《中华检验医学杂志》2008年第2期200-203,共4页Chinese Journal of Laboratory Medicine

基　　金：教育部博士点基金资助项目（20030246060）;上海市青年科技启明星计划资助项目（04QMX1408）

摘　　要：目的评价色素上皮细胞衍生因子（PEDF）对胶质瘤侵袭的抑制作用。方法将PEDF（100ng／m1）加入胶质瘤细胞U87中（U87PEDF），以未加PEDF蛋白的胶质瘤细胞作对照组（U87com），同时在U87PEDF中加入0．25μg／ml的促血管生成因子（VEGF，U87PEDF＋VEGF）。细胞迁徙实验检测PEDF抑制胶质瘤细胞侵袭性；实时荧光定量逆转录（RT）-PCR检测层粘连蛋白Laminin-8表达。结果迁徙实验结果表明，对照组胶质瘤细胞穿透数明显多于PEDF处理组细胞，加入VEGF后，胶质瘤细胞迁徙率的抑制率仍减少38％；实时荧光定量RT-PCR结果显示，U87对照组Laminin-8α4、β1、γ1mRAN表达分别为（1．043±0．090）、（0．823±0．012）、（0．762±0．005）拷贝／μl，明显高于U87mF组[（0．633±0．004）、（0．442±0．005）、（0．424±0．002）拷贝／μl，P〈0．05]。结论在VEGF存在的条件下，PEDF能有效抑制胶质瘤细胞的迁徙，表明其可能由下调Laminin-8基因的表达所致。Objective To assess the impact of pigment epithelium-derived factor （PEDF） on the migration of the glioma cells. Methods PEDF （ 100 ng/ml） was added to U87 cells（U87PEDF） , and VEGF of 0. 25μg/ml was added to U87PEDF＋VEGF while U87 cells as control（U87con）. The cell migratory assay was used for evaluating its inhibitory migration rate of PEDF. Real time RT-PCR was used for evaluating the expression of Laminin-8 gene. Results The number of migratory cells was higher than those with added PEDF, and the inhibitory rate of migratory was 38% even in the presence of VEGF. Real time RT-PCR revealed that the mRNA expression levels of α4,β1 ,γ1 were （ 1. 043±0. 090） , （0. 823 ±0. 012） , （0. 762 ±0. 05） copy / p,1, which were higher than those treated by PEDF （0. 633 ± 0. 004 ）, （ 0. 442 ±0. 005 ）, （0.424±0.002） copy/p,1, respectively （P 〈0.05）. Conclusion PEDF could decrease the migratory capacity of the glioma cells even in the presence of VEGF because of the regulation of Laminin-8 in part.

关 键 词：神经胶质瘤 肿瘤侵润 眼蛋白质类 神经生长因子 舍平类 

分 类 号：R686[医药卫生—骨科学]