不同渗透压及山梨醇浓度对猪体外受精卵早期发育的影响  

Effects of different osmotic pressures and sorbitol concentrations on subsequent embryonic development of porcine oocytes in vitro fertilization

作　　者：兰宗宝[1] 王修文[1] 何若钢[2] 卢晟盛[1] 张玲[1] 房慧伶[2] 蓝海恩 黄敏瑞 卢克焕[1] 

机构地区：[1]广西亚热带生物资源保护利用重点实验室,南宁530004 [2]广西大学动物科学技术学院,南宁530004 [3]广西畜牧研究所,南宁530001

出　　处：《广西农业科学》2008年第1期89-92,共4页Guangxi Agricultural Sciences

基　　金：广西科学研究与技术开发计划项目(桂科攻0330004-14);广西科学基金项目(桂科青0447004)

摘　　要：探讨胚胎培养液中不同渗透压及山梨醇浓度对猪体外受精卵早期发育的影响。结果表明，用NaCl调节渗透压时，猪体外受精卵的囊胚率随着渗透压的升高而增加，当渗透压为320-335mOsm时囊胚率最高；而用山梨醇调节渗透压时，以1．8mg／mL山梨醇调节渗透压至270-285mOsm的效果较好；同一渗透压水平下，培养液中添加山梨醇与未添加的处理组间差异不显著。说明本研究条件下，适当提高胚胎培养液渗透压有利于猪体外受精卵的早期发育。In the study, the effects of different osmotic pressures and sorbitol concentrations in embryo culture medium on subsequent embryonic development of porcine cocytes in vitro fertilization were investigated and discussed. The results indicated that when osmotic pressure of embryo culture medium was adjusted by NaCl, the blastocyst rate of porcine cocytes in vitro fertilization was increased with the increase of osmotic pressure, and the blastocyst rate was the highest when osmotic pressure was 320-335 mOsm. It had a better effect on blastocyst rate with osmotic pressure 270-285 mOsm adjusted by 1.8mg/mL sorbitol. Under the same level of osmotic pressure, whether adding sorbitol in culture medium or not, there was no significant difference in blastocyst rate. Therefore, under this experiment oondition, increasing the osmotic pressure of embryo culture medium would be suitable for the subsequent embryonic development of porcine oocytes in vitro fertilization.

关 键 词：猪卵母细胞 体外受精 胚胎培养 渗透压 山梨醇 

分 类 号：S828.35[农业科学—畜牧学]