盾叶薯蓣中甾体皂苷的分离与结构鉴定  

Isolation and structure identification of steroidal saponin from Dioscorea zingiberensis

作　　者：程娟[1] 胡长鹰[2] 庞自洁[3] 徐德平[1] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]暨南大学食品科学与工程系,广东广州510520 [3]江苏省、中国科学院植物研究所,江苏南京210014

出　　处：《中草药》2008年第2期165-167,共3页Chinese Traditional and Herbal Drugs

摘　　要：目的对盾叶薯蓣进行化学成分研究。方法利用反相色谱技术分离盾叶薯蓣水相部分中的化学成分,用1H-NMR、13C-NMR、135DEPT、HMQC和HMBC等方法鉴定其结构。结果从新鲜的盾叶薯蓣根茎分得3个甾体皂苷,分别鉴定为薯蓣皂苷元-3-O-β-D-吡喃葡萄糖基(1→3)-β-D-吡喃葡萄糖基(1→4)-[α-L-吡喃鼠李糖基(1→2 )]-β-D-吡喃葡萄糖苷(Ⅰ)、(25R)-26-O-β-D-吡喃葡萄糖基-呋甾烷-5-烯-3β,22ζ-二醇-3-O-β-D-吡喃葡萄糖基(1→3)-β-D-吡喃葡萄糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-吡喃葡萄糖苷(Ⅱ)、(25R)-26-O-β-D-吡喃葡萄糖基-呋甾烷-5-烯-3β,22ζ-二醇-7-羰基-3-O-β-D-吡喃葡萄糖基(1→3)-β-D-吡喃葡萄糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-吡喃葡萄糖苷(Ⅲ)。结论化合物Ⅲ为一新甾体皂苷,命名为盾叶薯蓣皂苷H(zingiberenin H)。Objective To study the chemical constituents of Dioscorea zingiberensis. Methods The fresh rhizome of D. zingiberensis was extracted three times, 3 h once with EtOH-H2O at 80℃. The EtOH was evaporated under reduced pressure to give a residue which was suspended in water and then was exacted with petroleum ether, ethyl acetate, and n-butanol fraction. The water fraction was isolated by the re versed-phase ODS column chromatography. The chemical structures were elucidated by means of 1H-NMR, 13C-NMR, ^135DEPT, HMQC, and HMBC spectroscopic nalyses. Results Three steroidal saponins were isolated from the fresh rhizome of D. zingiberensis. The compounds were identified as ： dios- genin- 3-O-β-D-glucopyranosyl （ 1→3 ）-β-D-glucopyranosyl （ 1 → 4 ）- [α-L-rhamnopyranosyl （ 1 →2 ） ]-β-D- glucopyranoside （Ⅰ）, （25R）-26-O-β-D-glucopyranosyl-furost-5-en-3β, 22ζ-diol 3-O-β-D-glucopyranosyl （ 1→3 ）-β-D-glucopyranosyl （1 →4 ）-[α-L-rhamnopyranosyl （ 1 → 2 ）]-β-D-glucopyranoside （ Ⅱ ）, and （25R）-26-O-β-D-glucopyranosyl-furost-5-en-3β, 22ζ-diol-7-carbonyl-3-O-β-D-glucopyranosyl （1→3）-β-D- glucopyranosyl （1→4）-[α-L-rhamnopyranosyl （1→2）]-β-3-D-glucopyranoside （Ⅲ）. Conclusion Compound Ⅱ is a novel compound named as zingiberenin H.

关 键 词：盾叶薯蓣 呋甾烷皂苷 甾体皂苷 盾叶薯蓣皂苷H 

分 类 号：R284.1[医药卫生—中药学]