均匀设计法优选金匮肾气丸中苷类成分的提取工艺  

Optimization of extracting technique for glycosides in Jinkui Shenqi Pills by uniform design

作　　者：李文兰[1] 任晓蕾[1] 赵稷[1] 季宇彬[1] 徐栋[1] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨150076

出　　处：《中草药》2008年第2期199-202,共4页Chinese Traditional and Herbal Drugs

基　　金：黑龙江省科技厅攻关项目(GC05C31601);黑龙江省教育厅振兴老工业基地项目(1151gzd25)

摘　　要：目的优选金匮肾气丸中苷类成分的提取工艺。方法采用HPLC法测定马钱苷、芍药苷的量,紫外分光光度法测定总苷和总皂苷的量。以浸膏率、马钱苷、芍药苷、总苷和总皂苷的量为指标,按U9(95)均匀设计表设计试验,分别考察乙醇体积分数、液固比、煎煮时间和煎煮次数4个因素的影响。结果通过DPS软件分析,得出苷类成分的最佳提取工艺为用14倍量58%乙醇煎煮3次,每次3h,在此工艺提取得马钱苷和芍药苷的量分别为2.4770、0.7684mg/g,以马钱苷计总苷的量为34.1782mg/g,以人参皂苷Re计总皂苷的量为4.9108mg/g。结论均匀设计法优选出的金匮肾气丸中苷类成分的提取工艺合理可行,为金匮肾气丸的进一步研究提供参考。Objective To optimize the extracting process of glycosides in Jinkui Shenqi Pills. Methods The extracting process of Jinkui Shenqi Pills was optimized by U9（9^5） uniform design with extract rate, loganin, paecniflorin, total glycosides, and total saponins, as indexes to observe the effect of ethanol con- centration, amount of extraction solvent added, duration of decoctions, and times of decoction. HPLC and UV-spectrophometer were used to determine the loganin, paecniflorin as well as the total glycosides and total saponins. Results Through the DPS software analysis, the most favorable conditions are： glycosides in Jinkui Shenqi Pills were extracted in 58% ethanol 14 times as much as it for 3 times, 3 h once. Using this extracting process, the obtained contents of loganin, paecniflorin, total glycosides, and total saponins were 2. 477 0 mg/g, 0. 768 4mg/g, 34. 178 2 mg/g, and 4. 910 8mg/g, respectively. Conclusion The extracting technique for glycosides in Jinkui Shenqi Pills by uniform design is suitable and feasible. The study could provide the references for determination and extraction of saponins in Jinkui Shenqi Pills and groundwork for its further investigation.

关 键 词：金匮肾气丸 苷类 均匀设计 提取工艺 

分 类 号：R284.2[医药卫生—中药学] R286.02[医药卫生—中医学]