利用茎尖离体嫁接获得大蒜体细胞嵌合体  

Obtaining Somatic Chimeras by in Vitro Meristem-apex Grafting-culture in Garlic(Allium sativum L.)

作　　者：徐启江[1] 陈青奇[2] 陈典[2] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040 [2]东北农业大学园艺学院

出　　处：《园艺学报》2008年第1期65-70,共6页Acta Horticulturae Sinica

基　　金：黑龙江省‘十五’攻关项目(GC01B315)

摘　　要：以‘阿城紫皮’大蒜和日本‘福地六瓣’白皮大蒜鳞茎茎尖为接穗、鳞茎盘为砧木,利用离体茎尖微体嫁接技术,研究了大蒜体细胞嵌合体的培育,用RAPD分子标记技术进行了嵌合体的鉴定。结果表明:最佳生芽培养基为MS+NAA 0.1 mg·L^(-1)+2ip 0.4 mg·L^(-1);最佳生根培养基为1/2 MS+IBA 1.5 mg·L^(-1)。嫁接成活率达16.00%～26.67%。利用引物OPF-16鉴定表明,白/紫、白·紫/紫、白·紫/白、紫/白4种嫁接方式获得了嵌合体。利用引物OPJ-12鉴定表明,紫/白嫁接方式获得了嵌合体,而引物OPE-05鉴定未能检测出亲本与嫁接苗之间的差异。An efficient chimera formation method by tissue culture combined with grafting was studied in garlic （Allium sativum L. ）. Garlic cultivars ‘Acheng purple＇ garlic and Japanese‘ Fukuchi＇ white garlic were used for intervarietal chimera formation. The stem discs were used as stock. The meristem-apexs of these cultivars bulb were used as scions. The Chimeric shoots were examined by RAPD （random amplified polymorphic DNA）. The results showed that the optimized medium for shooting was MS ＋ NAA 0. 1 mg · L^-1 ＋ 2ip 0. 4 mg · L^-1 , and that for rooting was 1/2 MS ＋ IBA 1.5 mg · L^-1. Chimeric shoots were obtained by grafted culture method on these media, resulting in 20% formation. The result of RAPD - PCR using primer OPF- 16 indicated that four grafting combination, i.e. , white/purple, white purple/purple, white purple/white, and purple/white, produced .chimeras. The result of RAPD - PCR using primer OPJ-12 indicated that grafting combination of purple/white produced chimeras. Primer OPE-05 did not give a significant result.

关 键 词：大蒜 离体 嫁接 嵌合体 RAPD 

分 类 号：S633.4[农业科学—蔬菜学]