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机构地区:[1]中国科学院植物研究所
出 处:《园艺学报》2008年第1期94-98,共5页Acta Horticulturae Sinica
基 金:中国科学院创新工程重要方向性项目(KSCX2-YW-N-52);科技部平台项目(2005DKA21006)
摘 要:研究了西南凤尾蕨的发育过程和孢子的无菌培养方法。结果如下:孢子萌发为书带蕨型,配子体发育为水蕨型。1/2MS培养基中萌发率可达83.3%;MS培养基中配子体成苗率可达55.4%;试管苗移载成活率可达80%以上。认为利用孢子的无菌繁殖方法进行西南凤尾蕨的规模化生产具有可行性。The development process and spore sterile culture of Pteris wallichiana Agardh. were studied respectively. The results showed that the spore germination was of the Vittaria-type while the gametophyte development was of the Ceratopteris-type. The maximum germination rate was obtained in 1/2MS medium, which reached 83.3% ; the highest rate of gametophyte to sporophyte was achieved in MS medium, which reached 55.4% ; the transplant survival rate of sporophyte in vitro reached above 80%. The research indicates that the utilization of the spore Sterile culture of P. wallichiana for its large-scale production is possible.
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