硝酸钙处理对菊花扦插生根及抗氧化酶活性的影响  

Effects of Ca^(2+) on Antioxidant Enzyme Activities During Rooting of Chrysanthemum Cuttings

作　　者：郑成淑[1] 王文莉[1] 孙宪芝[1] 梁芳[1] 张翠华[1] 

机构地区：[1]山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安271018

出　　处：《园艺学报》2008年第2期263-268,共6页Acta Horticulturae Sinica

基　　金：国家‘十一五’科技支撑计划项目(2006BAD10B07);山东农业大学青年科技创新项目(23408)

摘　　要：以切花菊品种‘万盛’为材料,研究了不同浓度Ca(NO3)2处理对菊花扦插生根过程中插穗叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性以及膜质过氧化产物丙二醛(MDA)含量的影响。结果表明,60mg·L-1Ca2+处理的生根数最多,并且插穗鲜样质量和干样质量也比对照明显增加,而80mg·L-1Ca2+处理的生根数、插穗鲜样质量和干样质量以及生根率比对照减少。60mg·L-1Ca2+处理的SOD、POD、CAT和APX活性比其它处理显著增加,MDA含量明显减少。以上结果表明,在菊花扦插过程中,采用适当浓度的Ca2+处理可以提高插穗叶片SOD、POD、CAT和APX等保护酶的活性和降低MDA含量,从而促进菊花扦插生根,并提高扦插苗品质。Effects of Ca （ NO3 ） 2 under different concentrations on rooting and activities of superoxide dismutase （SOD） , peroxidase （POD） and catalase （CAT） and ascorbate peroxidase （APX） , and malondial- dehyde （MDA） content in the leaves of cuttings of Dendrandema grandiflora ‘ Wansheng＇ were studied. The results showed that the root number was the highest in 60 mg · L^-1Ca^2＋ treated cuttings with significant increase in fresh and dry mass of the cuttings compared to the control. But root number, fresh and dry mass and rooting rates decreased at 80 mg · L^-1Ca^2＋ . In 60 mg· L^-1Ca^2＋ treated plants, the activities of SOD, POD, CAT and APX increased and MDA content decreased significantly compared with other plants. These results indicated that Ca^2＋ treatments at proper dosage might enhance rooting of cuttings by increasing the activities of antioxidant enzymes and decreasing membrane lipid peroxidation during rooting in the cuttings of D. grandiflora.

关 键 词：菊花 钙 扦插 生根 抗氧化酶 丙二醛 

分 类 号：S682.11[农业科学—观赏园艺]