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出 处:《作物杂志》2008年第1期21-25,共5页Crops
摘 要:玉米杂种优势群及其模式的研究对于提高育种准确性和预见性具有重要意义。本研究以我国主要玉米杂交种的亲本为材料,利用SSR标记的遗传距离划分优良玉米种质的杂种优势群。选用的110对SSR引物对30份温带玉米自交系进行分析,共检测到546条带,表明该30份自交系在分子标记水平上具有较丰富的遗传多样性,其PIC变异范围在0.1115~0.91之间,平均为0.652,大部分集中在0.5~0.8之间。根据SSR分析结果计算30个自交系之间遗传距离并进行聚类分析,可将30份自交系划分为7个杂种优势群:第一类是P类群,有P007、齐319、丹599、沈137、178、P138和87—1共7个自交系;第二类是以黄早四等为代表的唐四平头群,共6个系,有K12、Q1261、黄野四一3、黄早四、冀35和昌7—2;第三类是Reid群,有478、B73、K14、黄C和沈5003共5个自交系;第四类是以自330等为代表的自330群,共有4个自交系,包括中综3、中综31、自330和丹340;第五类是E28群,包括E28以及二环系一郑22;第六类是Lancaster群,包括M017、荻唐黄17和齐205共3个自交系;此外,鲁原92、吉63、旅28被划为一群,为其他群。It is necessary for improving the accuracy and predictability of maize breeding to study heterotic grouping of maize and its parents. In this study, using the main maize hybrids in China as materials, the heterotic groups of maize were distinguished by the genetic distance with the SSR marker. 546 polymorphic amplified fragments were detected among the 30 maize inbred lines with the 110 SSR primers, where variations in PIC ranged from 0. 1115 to 0. 91 (average of 0. 652) and concentrated on 0. 5 -0. 8, suggesting that these inbred lines held great genetic deviations at molecule level. Based on the results of SSR analysis, the 30 inbred lines could be distinguished into seven types, that is, ( 1 ) P type, including seven inbred lines : P007, Qi319, Dan599, Shen137,178, P138,87-1 ; (2) Tangsipingtou type,including six inbred lines : K12, Q1261, Huanyesi-3, Huangzao4, Ji35, Chang7-2 ; ( 3 ) Reid type, ineluding five inbred lines :478, B73, K14, Huang C, Shen5003 ; (4) Zi330 type, including four inbred lines : Zhongzong3, Zhongzong31, Zi330,340 ; (5) E28 type, including E28 and Zheng22 ; (6) Lancaster type, including Mo17, Ditanghuangl7 and Qi205 ;and (7) the others,including Luyuan92 ,Ji63 ,Lv28.
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