C6／36浓核病毒样颗粒在大肠杆菌中的表达与组装  

Expression and Assembly of C6/36 Densovirus-like Particles in E.coli

作　　者：李志红[1] 张景强[2] 

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]中山大学有害生物控制与资源利用国家重点实验室,广州510275

出　　处：《三峡大学学报（自然科学版）》2008年第1期97-100,共4页Journal of China Three Gorges University：Natural Sciences

基　　金：国家自然科学基金资助项目(10274106);三峡大学博士科技启动基金资助项目

摘　　要：通过PCR获得C6/36细胞浓核病毒(C6/36DNV)的结构蛋白VP1和VP2的基因,经克隆表达,在大肠杆菌中得到了含6×His标签的VP1或VP2的融合蛋白表达产物;两种表达产物经超速离心纯化后,在电镜下均观察到了大小(约25 nm)和形态都与野生C6/36DNV相似的病毒样颗粒(VLPs),且两种VLPs(VP1-VLPs和VP2-VLPs)在电镜下未见有明显差别,说明VP1和VP2在大肠杆菌中表达后均可独立组装成VLPs.The genes encoding two structural protchas（VP1 and VP2） of C6/36 cell densovirus （C6/36DNV） were amplified by PCR and cloned into the vector. After induced with IPTG, the 6xHis-VP1 and 6xHis-VP2 fusion proteins were expressed in E. coli. After the products were purified by ultracentrifugation, two kinds of VLPs, VP1-VLPs and VP2-VLPs, which were similar to native C6/36DNV virions in size （25nm in diameter） and shape, were observed under electron microscope. No distinguished difference was observed between these VLPs. The results show that VP1 and VP2 are able to form VLPs after expressed in E. coli.

关 键 词：C6/36DNV 表达 组装 VLPS 

分 类 号：S476.13[农业科学—农业昆虫与害虫防治]