来氟米特活性代谢产物A771726对人外周血T淋巴细胞的免疫调节作用  

Immunoregulatory effects of leflunomide on human peripheral of T-lymphocytes

作　　者：裘影影[1] 李晶[1] 汤郁[1] 尤海燕[1] 朱伟[2] 焦志军[1] 

机构地区：[1]江苏大学附属医院风湿科,江苏镇江212001 [2]江苏大学医学技术学院,江苏镇江212013

出　　处：《江苏大学学报（医学版）》2008年第1期23-26,29,共5页Journal of Jiangsu University：Medicine Edition

基　　金：江苏省科技厅社会发展基金资助项目(BS2005043)

摘　　要：目的:探讨来氟米特(leflunomide,LEF)在体外对人外周血T淋巴细胞的免疫调节作用。方法:在植物血凝素(phytohemagglutinin,PHA)刺激下,人外周血淋巴细胞与不同剂量的LEF(5,15,45μg/ml)共培养。检测各组T淋巴细胞的增殖、凋亡情况,分析各组T淋巴细胞免疫表型CD152,CD28的表达以及各组T淋巴细胞的IFN-γ,IL-10,TGF-β1 mRNA水平。结果:在体外,LEF对由PHA诱导的T淋巴细胞的增殖、凋亡均有抑制作用,且抑制作用与LEF呈剂量依赖性。LEF对T淋巴细胞CD152,CD28的表达无明显影响。LEF能促进IL-10,TGF-β1 mRNA的表达,抑制IFN-γmRNA的表达。结论:LEF可能通过抑制T淋巴细胞增殖、减少T淋巴细胞凋亡、促进T淋巴细胞IL-10、TGF-β1 mRNA表达及抑制IFN-γmRNA表达来下调免疫反应。Objective： To investigate the immunoregulatory effects of leflunomide （ ripheral of T-lymphocytes in vitro. Methods： The LEF （5,15,45 μg/ml） were added lymphocytes from unrelated donors in the presence of phytohemagglutinin（PHA）. We LEF） on human peinto wells containing assessed the proliferation of T-lymphocytes. The immunophenotypes （ CD152, CD28 ） of T-lymphocytes were analyzed by flow cytometry. We also observed the T lymphocytes＇ apoptosis and the mRNA expression of interferon-γ （IFN-γ）, interleukin-10 （IL-10）, transforming growth factor-β1 （TGF-β1）. Results： In all the experiments, with the presence of LEF human peripheral T-lymphocytes resulted in a statistically significant decrease of their proliferative activities and apoptosis as dose-dependent manner. No inhibitory effects on CD152 and CD28 of T-lymphocytes had been observed. LEF promote the mRNA expression of IL-10, TGF-β1 and inhibit IFN-γ. Conclusion： LEF can inhibit the proliferative activities and apoptosis of human peripheral T cells and promote the mRNA expression of T-lymphocytes＇ IL-10, TGF-β1 and can lower the mRNA expression of IFN-γ, which may play an important role in its immunoimpressive affects.

关 键 词：来氟米特 T淋巴细胞 免疫调节 

分 类 号：R593[医药卫生—内科学]