Ⅱ型胶原蛋白抗血清的制备和检测  

Preparation and detection of antisera for collagen protein typeⅡ

作　　者：姜旭淦[1] 朱敏娟[1] 葛彦文[1] 吴亮[1] 陈盛霞[1] 许化溪[1] 

机构地区：[1]江苏大学医学技术学院,江苏镇江212013

出　　处：《江苏大学学报（医学版）》2008年第1期34-36,39,共4页Journal of Jiangsu University：Medicine Edition

基　　金：江苏省高校自然科学指导性计划项目(06KJD310036);江苏大学第六批学生科研课题(06A091)

摘　　要：目的:探讨Ⅱ型胶原蛋白(collagen protein typeⅡ,CⅡ)抗体产生规律,制备CⅡ抗血清。方法:采用纯化的鸡和猪CⅡ免疫新西兰白兔,每隔10 d收集血清,以改良间接ELISA法检测抗CⅡ抗体效价。结果:鸡CⅡ免疫新西兰白兔后,第10天抗体效价为1∶3 200,第20天升至1∶204 800;加强免疫后,第30天和第40天抗体效价分别达到1∶409 600和1∶819 200。猪CⅡ免疫新西兰白兔后,第10天血清中未能检测出抗体,第20天血清抗体效价为1∶3 200;加强免疫后,第30天和第40天抗体效价分别为1∶51 200和1∶102 400。结论:采用纯化的鸡和猪CⅡ免疫新西兰白兔后,经一定潜伏期血清中出现抗体,可以获得高效价抗CⅡ抗体血清。抗体产生情况符合机体初次及再次免疫应答的有关规律,但血清抗体产生的潜伏期和效价与不同种属来源的胶原蛋白有关。Objective： To investigate the regular patterns of antibody production and prepare the antiserum on collagen protein type Ⅱ （ C Ⅱ ）. Methods： The New Zealand white rabbits were immunized with pudried chicken and pig C Ⅱ and then the rabbit sera were collected every 10 days. The improved indirect ELISA method was adopted to detect the titers of anti-C Ⅱ antibodies. Results： When the rabbits were immunized with purified chicken C Ⅱ , the antibody titers in sera were 1 ： 3 200 at 10 th day and 1 ： 204 800 at 20th day. After booster the antibody titers increased to 1：409 600 at 30 th day and 1：819 200 at 40 th day. When the rabbits were immunized with purified pig C Ⅱ , the antibodies could not be detected at 10 th day. But at 20 th day the antibody titer was 1 ： 3 200. After booster the antibody titers were then increased to 1 ： 51 200 at 30 th day and 1：102 400 at 40 th day. Conclusion： The anti-C Ⅱ antibodies can emerge in serum after latency and the antiserum with high titer can be obtained when the rabbits were immunized with purified chicken and pig CⅡ. The antibody production was consistent with relevant regularity of primary and secondary immune responses. The latent phases and titers of anti-CⅡ antibodies in sera were concerned with collagen proteins from different genus.

关 键 词：鸡 猪 Ⅱ型胶原蛋白 抗Ⅱ型胶原蛋白抗体 酶联免疫吸附实验 

分 类 号：R446.6[医药卫生—诊断学]