载脂蛋白E基因多态性与早发冠心病遗传关系研究  

Analysis of relationship between apolipoprotein E gene polymorphism and early onset of coronary heart disease

作　　者：丁福祥[1] 刘华欣[2] 刘同库[1] 

机构地区：[1]北华大学附属医院,吉林吉林132011 [2]第四军医大学吉林军医学院,吉林吉林132013

出　　处：《中国热带医学》2008年第1期41-42,共2页China Tropical Medicine

摘　　要：目的探讨载脂蛋白E(apoE)基因多态性与早发冠心病(CHD)的关系。方法应用聚合酶链-限制性片段长度多态性(PCR-RFLP)方法先扩增出apoE基因第4外显子内的227bp片段,继以限制性内切酶CfoI酶解消化,8%聚丙烯酰胺凝胶电泳,最后银染显带判定基因型,并计算等位基因频率。共检测了52例早发冠心病患者和110例正常对照者的apoE基因型及等位基因频率。结果早发冠心病患者apoEε4/3基因型和ε4等位基因频率显著高于正常对照组(P<0.05)。结论apoE基因多态性与早发冠心病的发生有关。该检测方法快速、准确、分辨率高。Objective To investigate the relationship between appolipoprein E （apoE） gene polymorphism and early - onset coronary heart disease. Methods The 277 base DNA fragment in the forth exon of apoE gene was amplified by using polymerase chain reaction- restriction fragmant length polymorphism and then digested with restriction enzyme CfoI, eleetrophoresis on 8% polyacrylamaid gel for the cleavaged products, subsequently genotype was determined by silver staining and allele frequencies was analyzed in 52 eases with early - onset coronary heart disease and 110 healthy controls. Results The hererozygous genotyping apoEε4/3 and the frequencies ofε4 allele in early - onset coronary heart disease group were significantly higher than that in age - matched controls （P 〈 0.05）. Conclusion The occurrence of early- onset CHD is affected by the apo E gene polymorphisms.

关 键 词：载脂蛋白E 基因多态性 冠心病 

分 类 号：R541.4[医药卫生—心血管疾病]