维拉帕米对人视网膜色素上皮细胞增殖的抑制作用  

The Inhibitory Effect of Verapamil on Proliferation of Retinal Pigment Epithelial Cells of Human

作　　者：李劲 宋琳[2] 龚荃[3] 李贵刚[2] 王军明[2] 肖诗艺 

机构地区：[1]暨南大学医学院附属第二医院、深圳市人民医院眼科,深圳518020 [2]华中科技大学同济医学院附属同济医院眼科,武汉430030 [3]武汉生物制品研究所,武汉430060

出　　处：《华中科技大学学报（医学版）》2008年第1期117-120,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的评价钙通道拮抗剂维拉帕米(verapamil)对人视网膜色素上皮(RPE)细胞增殖的影响。方法在原代培养的人视网膜色素上皮(RPE)细胞中分别加入不同浓度的维拉帕米溶液(0.1、1、1×101、1×102、1×103、1×104μmol/L),于培养72、96和120h后用四甲基偶氮唑蓝(MTT)比色法,在酶标仪上测630nm处吸光度值,与无血清培养液对照组比较,计算不同条件下维拉帕米对细胞增殖的抑制率。结果维拉帕米在1×10-1～1×104μmol/L浓度范围内对RPE的细胞增殖有明显抑制作用,此抑制作用具有浓度依赖性。维拉帕米在细胞培养72、96和120h后,对人RPE细胞增殖的半数抑制浓度(IC50)分别为10.35、5.92和3.85μmol/ml。结论维拉帕米对体外培养的原代人RPE细胞的增殖有抑制作用,具有预防和治疗增殖性玻璃体视网膜病的潜在价值。Objective To evaluate the effects of calcium channel blocker, verapamil on proliferation of retinal pigment epithelial cells（RPECs） of human in vitro. Methods RPECs of human were treated with different concentrations of verapamil （0.1, 1, 10, 10^2 , 10^3 , 10^4 /μmol/L）. After culture for 72, 96 and 120 h, the absorbance（A） values of the solution were determined by MTT method at 630 nm and compared with those in the control group. The proliferation rate at different concentrations of verapamil was calculated. Results The results showed that the concentrations between 1 × 10^-1 - 1 × 10^4 μmol/L of verapamil could inhibit the proliferation of RPECs significantly in a dose-dependent manner. Verapamil IC50 for RPECs was 10.35, 5.92 and 3.85 /μmol/ml at different time points （72, 96 and 120 h）. Conclusion These data showed verapamil could inhibit the proliferation of RPECs and suggested that it might be used as a medicine for preventing and treating vitreoretionopathy in clinical practice.

关 键 词：钙通道拮抗剂 维拉帕米 视网膜色素上皮细胞 增殖性玻璃体视网膜病变 

分 类 号：Q593.9[生物学—生物化学]