检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:谢模英[1] 鲁文果[1] 陈红[1] 杨慧民[1] 张苏明[1]
出 处:《华中科技大学学报(医学版)》2008年第1期136-138,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基 金:国家自然科学基金资助项目(No30370505)
摘 要:目的研究Duchenne肌营养不良模型鼠(mdx鼠)局部肌肉内鼠胚胎干细胞(ESCs)移植治疗后,ESCs的分化、肌肉病理改变及dystrophin蛋白表达变化。方法eGFP标记的ESCs局部肌肉注射移植入mdx鼠,4周后观察核内移及dystrophin蛋白表达变化。结果移植后4周mdx鼠核内移改善不明显,dystrophin蛋白阳性表达及肌纤维增加均不明显。结论局部肌肉内胚胎干细胞移植治疗mdx鼠效果不明显。胚胎干细胞移植后分化为骨骼肌细胞的比例低。Objective To investigate the differentiation of embryonic stem cells (ESCs), the muscular pathological changes and the expression of dystrophin protein after transplantation of mice ESCs into mdx mice skeletal muscle. Methods ESCs labeled with effectively green fluorescence protein (eGFP) were injected into the hindleg gastrocnemius muscle of mdx mice. Four weeks after implantation, differentiation and central nuclei in myofibers and dystrophin protein expression were analyzed. Resuits Four weeks after implantation, ESCs did not differentiate into muscle cell, and there were no significant changes in the central nuclei in myofibers and dystrophin protein expression in mdx mice. Conclusion The curative effects of ESCs transplantation by local muscular injection for mice with mdx were not satisfactory. The proportion of skeletal muscles differentiated from the transplanted ESCs was low.
分 类 号:R329.2[医药卫生—人体解剖和组织胚胎学]
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.200