Construction of a BAC library from cucumber (Cucumis sativus L.) and identification of linkage group specific clones  

作　　者：Yuan Guan Qi Chen Junsong Pan Zheng Li Huanle He Aizhong Wu Rentao Song Run Cai 

机构地区：[1]School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China [2]School of Life Sciences, Shanghai University, Shanghai 200444, China

出　　处：《Progress in Natural Science:Materials International》2008年第2期143-147,共5页自然科学进展·国际材料（英文版）

基　　金：2007 National Natural Science Foundation of China ; Chinese Academy of Sciences. National Natural Science Foundation of China （Grant No. 30671111）;Shanghai Municipal Scientific ＆ Technological Commission （Grant No. 043919317） ; Shanghai Leading Academic Discipline Project （B209）.

摘　　要：A bacterial artificial chromosome （BAC） library consisting of 19,200 clones with an average insert size of 105 kb has been constructed from a cucumber （Cucumis sativus L.） inbred line S94, derived from a cultivar in North China. The entire library was equivalent to approximately 5 haploid cucumber genomes. To facilitate chromosome engineering and anchor the cucumber genetic linkage map to its chromosomes, 15 sequence-characterized amplified regions （SCAR） and seven simple sequence repeats （SSR） markers from each linkage group of cucumber were used to screen an ordered array of pooled BAC DNA with polymerase chain reaction （PCR）. Fifteen markers gave at least two positive clones. As a result, 22 BAC clones representing 7 linkage groups of cucumber were identified, which further validated the genome coverage and utility of the library. This BAC library and linkage group specific clones provide essential resources for future research of the cucumber genome.A bacterial artificial chromosome (BAC) library consisting of 19,200 clones with an average insert size of 105 kb has been constructed from a cucumber (Cucumis sativus L.) inbred line S94,derived from a cultivar in North China. The entire library was equivalent to approximately 5 haploid cucumber genomes. To facilitate chromosome engineering and anchor the cucumber genetic linkage map to its chromosomes,15 sequence-characterized ampli?ed regions (SCAR) and seven simple sequence repeats (SSR) markers from each link-age group of cucumber were used to screen an ordered array of pooled BAC DNA with polymerase chain reaction (PCR). Fifteen mark-ers gave at least two positive clones. As a result,22 BAC clones representing 7 linkage groups of cucumber were identified,which further validated the genome coverage and utility of the library. This BAC library and linkage group specific clones provide essential resources for future research of the cucumber genome.

关 键 词：CUCUMBER BAC library Molecular markers PCR screening Specific clones 

分 类 号：S642.2[农业科学—蔬菜学]