Cloning and characterization of 37 kDa laminin receptor precursor in pearl oyster,Pinctada fucata  

作　　者：Yaopeng Fu Liping Xie Rongqing Zhang 

机构地区：[1]Institute of Marine Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China [2]Protein Science Laboratory of the Ministry of Education, Tsinghua University, Beijing 100084, China

出　　处：《Progress in Natural Science:Materials International》2008年第2期233-238,共6页自然科学进展·国际材料（英文版）

基　　金：2007 National Natural Science Foundation of China;Chinese Academy of Sciences;National Natural Science Foundation of China(Grant No.30530600);National High Technology Reserch and Development Program of China(Grant Nos.2006AA09Z413,2006AA09Z441).

摘　　要：A 1063 bp cDNA clone encoding a putative 37 kDa laminin receptor precursor （37 kDa LRP） is isolated from the mantle tissue of pearl oyster, Pinctadafucata. The amino acid sequence predicted from the cDNA sequence is 301 residues long, with a calculated molecular mass of 33.5 kDa. RT-PCR analysis shows that 37 kDa LRP mRNA is especially highly expressed in the mantle while widely expressed in several tissues. In situ hybridization analysis reveals that 37 kDa LRP is expressed in the outer epithelial ceils of the mantle edge, suggesting its involvement in cell proliferation and secretion in P. fucata. The identification and characterization of 37 kDa LRP in the pearl oyster will help us to further understand the signal transduction in the processes of mantle epithelial cell proliferation and tissue formation.A 1063 bp cDNA clone encoding a putative 37 kDa laminin receptor precursor (37 kDa LRP) is isolated from the mantle tissue of pearl oyster,Pinctada fucata. The amino acid sequence predicted from the cDNA sequence is 301 residues long,with a calculated molec-ular mass of 33.5 kDa. RT-PCR analysis shows that 37 kDa LRP mRNA is especially highly expressed in the mantle while widely expressed in several tissues. In situ hybridization analysis reveals that 37 kDa LRP is expressed in the outer epithelial cells of the mantle edge,suggesting its involvement in cell proliferation and secretion in P. fucata. The identification and characterization of 37 kDa LRP in the pearl oyster will help us to further understand the signal transduction in the processes of mantle epithelial cell proliferation and tissue formation.

关 键 词：37 kDa laminin receptor precursor Mantle epithelial cell Pearl oyster 

分 类 号：S96[农业科学—水产养殖]