脂微球前列腺素E_1治疗缺血性结肠炎的研究  

Lipo-PGE_1 in the treatment of ischemic colitis:an experimental study

作　　者：杨柳[1] 吴本俨[1] 顾瑛[2] 尤伟缔[1] 王卫华[1] 

机构地区：[1]解放军总医院南楼消化科,北京市100853 [2]解放军总医院南楼激光科,北京市100853

出　　处：《中华老年多器官疾病杂志》2008年第1期47-50,共4页Chinese Journal of Multiple Organ Diseases in the Elderly

摘　　要：目的研究脂微球前列腺素E1(Lipo-PGE1)对大鼠缺血性结肠炎(IC)治疗作用,并探讨其治疗机制。方法用光化学法建立大鼠IC模型,观察不同剂量Lipo-PGE1(0.5,1,2μg/kg,静滴,给药5d)治疗后结肠黏膜损伤指数(LI)、过氧化物歧化酶(SOD)、丙二醛(MDA)、6-酮-前列腺素F1α(6-keto-PGF1α)、血栓素B2(TXB2)及6-keto-PGF1α/TXB2(P/T)的变化,分析肿瘤坏死因子α(TNF-α)、细胞间黏附分子1(ICAM-1)在结肠组织的表达情况。结果与生理氯化钠溶液阴性对照组比较,1μg/kg和2μg/kg组LI均显著降低,SOD、P/T显著升高,而MDA和TXB2含量及TNF-α和ICAM-1的阳性细胞数均显著降低。结论Lipo-PGE1对IC有较好的治疗效果,可以减轻结肠损伤。治疗机制可能主要与抑制血栓形成、改善组织微循环、减轻炎症反应及氧自由基的产生有关。Objective To investigate the efficacy and mechanism of lipo-prostaglandin E1 （Lipo-PGE1） in the treatment of ischemic colitis（IC）. Methods IC model rats were divided into different groups and treated with different doses （0.5,1,2μg/kg） of Lipo-PGE1. The rats of control group were given physiologic saline. All rats were sacrificed after continuous treatmen for 5 d. Then the changes of colonic mucosa lesion index（LI）, SOD, MDA, 6-keto- PGF1α ,TXB2, 6-keto-PGF1α/TXB2 （P/T） and expression of TNF-α and ICAM-1 in control group and treatment groups were measured. Results In comparison with the control group,SOD activity and P/T increased significantly, while LI,MDA,TXB2, expression of TNF-α and ICAM-1 decreased significantly in 1 and 2μg/kg groups. Conclusion Lipo-PGE1 can decrease the IC rats＇ injury. The therapeutic mechanism may be inhibition of thrombogenesis, improving tissue microcirculation, lessening inflammatory reaction and oxygen free radical generation.

关 键 词：结肠炎 缺血性 光化学 前列地尔 

分 类 号：R543.31[医药卫生—心血管疾病] R574.62[医药卫生—内科学]