Heat and hyposmotic stimulation increase in [Ca^(2+)]_i by Ca^(2+) influx in rat synoviocytes  

作　　者：SUN WenWu HU Fen YANG WenXiu 

机构地区：[1]Biophysics Department in School of Physics, Key Laboratory of Bioactive Materials for Ministry of Education, Nankai University,Tianjin 300071, China

出　　处：《Chinese Science Bulletin》2008年第4期548-554,共7页

基　　金：Supported by a foundation of the City of Tianjin,China (Grand No. 983113411)

摘　　要：Rheumatoid arthritis(RA),which is marked by inflammatory synovitis,is a common,chronic autoim-mune-disease,whose pathogenesis is complex and still unclear.In order to explore the effects of heat and hyposmotic stimuli on synoviocytes in rheumatoid arthritis,the changes of [Ca2+]i induced by heat,hyposmotic and 4α-PDD stimuli were observed in synoviocytes.[Ca2+]i elevation induced by heat ≥ 28℃,hyposmotic and 4α-PDD stimuli is found to be positively relative to increasing temperature,decreasing osmolality and rising concentration of 4α-PDD.Results show that there is reciprocity among these stimuli and desensitization,and that [Ca2+]i elevation depends on Ca2+ influx,but not necessarily links to Ca2+ release from intracellular stores and voltage-dependent Ca2+ channel in synoviocytes.The above characteristics of Ca2+ influx are similar to those of TRPV4.A probable mechanism has been suggested that heat and hyposmotic stimulation might increase the level of [Ca2+]i by activating the TRPV4-like channel and Ca2+ influx in the synoviocytes.Rheumatoid arthritis （RA）, which is marked by inflammatory synovitis, is a common, chronic autoimmune-disease, whose pathogenesis is complex and still unclear. In order to explore the effects of heat and hyposmotic stimuli on synoviocytes in rheumatoid arthritis, the changes of [Ca^2＋]i induced by heat, hyposmotic and 4α-PDD stimuli were observed in synoviocytes. [Ca^2＋]i elevation induced by heat 28℃, hyposmotic and 4α-PDD stimuli is found to be positively relative to increasing temperature, decreasing osmolality and rising concentration of 4α-PDD. Results show that there is reciprocity among these stimuli and desensitization, and that [Ca^2＋]i elevation depends on Ca^2＋ influx, but not necessarily links to Ca^2＋ release from intracellular stores and voltage-dependent Ca^2＋ channel in synoviocytes. The above characteristics of Ca^2＋ influx are similar to those of TRPV4. A probable mechanism has been suggested that heat and hyposmotic stimulation might increase the level of [Ca^2＋]i by activating the TRPV4-like channel and Ca^2＋ influx in the synoviocytes.

关 键 词：风湿性关节炎 热扩散 低渗透性 钙离子 

分 类 号：R593.21[医药卫生—内科学]