Morphological and phylogenetic analysis of a Gymnodinium-like species from the Chinese Coast  

作　　者：CHEN GuoFu WANG GuangCe ZHANG ChunYun ZHOU BaiCheng 

机构地区：[1]Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China [2]College of Oceanology, Harbin Institute of Technology (Weihai), Weihai 264209, China [3]College of Oceanology, Tianjin University of Science and Techology, Tianijin 100022, China

出　　处：《Chinese Science Bulletin》2008年第4期561-567,共7页

基　　金：Supported by the National Natural Science Foundation of China (Grant Nos. 40476059, u0633006);Qingdao Scientific Foundation (BC3052407);Harbin Institute of Technology (Weihai)(HIT(WH)07)

摘　　要：A Gymnodinium-like species was studied with light microscopy(LM) and scanning electron microscopy(SEM).Also,the internal transcribed spacers(containing 5.8S rDNA) and large ribosomal subunit DNA(D1-D2) sequences were obtained by PCR amplification,and then sequenced to explore the rela-tionships within our isolate,Gymnodinium and other Gymnodinium-like species,including Karenia,Gyrodinium,Karlodinium and Symbiodinium.The LM observation showed that the species was characterized by moving in a levorotatory direction,visible hypocone,epicone and transverse groove,all of which are typical for Gymnodinium.In addition,two flagella could be found under SEM.The phylogenetic analysis revealed that the isolate grouped with Symbiodium,rather than other relevant dinoflagellates.All results showed our isolate belongs to Symbiodium.The strain was isolated from a red tideA Gymnodiniurn-like species was studied with light microscopy （LM） and scanning electron microscopy （SEM）. Also, the internal transcribed spacers （containing 5.8S rDNA） and large ribosomal subunit DNA （D1-D2） sequences were obtained by PCR amplification, and then sequenced to explore the relationships within our isolate, Gymnodinium and other Gymnodiniurn-like species, including Karenia, Gyrodinium, Karlodinium and Symbiodinium. The LM observation showed that the species was characterized by moving in a levorotatory direction, visible hypocone, epicone and transverse groove, all of which are typical for Gymnodinium. In addition, two flagella could be found under SEM. The phylogenetic analysis revealed that the isolate grouped with Symbiodium, rather than other relevant dinoflagellates. All results showed our isolate belongs to Symbiodium. The strain was isolated from a red tide water sample, denoting that Symbiodium may be causative species for algal bloom.

关 键 词：赤潮 海水污染 藻类 形态结构 系统发生学 

分 类 号：Q949.2[生物学—植物学] X55[环境科学与工程—环境工程]