生物检测用纳米金粒子还原制备方法比较  

Comparison on Reductive Preparations of Gold Nanoparticles in Biodetection

作　　者：尹晋津[1] 许利剑[1] 曾晓希[1] 汤建新[1] 

机构地区：[1]湖南工业大学绿色包装与生物纳米技术应用重点实验室,湖南株洲412008

出　　处：《湖南工业大学学报》2008年第1期104-108,共5页Journal of Hunan University of Technology

基　　金：国家863高技术资助项目(2006AA03Z357);中国博士后科学基金资助项目(20060390887);湖南省教育厅基金资助项目(06C262)

摘　　要：分别采用柠檬酸钠还原法、柠檬酸钠-鞣酸还原法、抗坏血酸还原法、硼氢化钠还原法4种方法制备了粒径为十几纳米的胶体金,并对制得的纳米胶体金粒径大小、分散度、稳定性及形貌进行了表征和比较分析。结果表明,采用柠檬酸钠-鞣酸还原法制备胶体金纳米粒子的过程相对简单,所得粒子平均直径约为十几纳米,粒径分布窄,形貌均一,稳定性和分散性好,满足生物标记探针的应用要求,相对其它3种方法具有较大优势。Colloidal gold was reductively prepared by sodium citrate, sodium citrate-tannin, ascorbic acid and sodium borohydride respectively. And corresponding gold particles size, morphology, dispersion, and stability of the samples were characterized and compared. The results showed that the colloidal gold particles via sodium citrate-tannin reduction method possessed a diameter range of ca. 10 nm, and demonstrated narrow size distribution, uniform morphology, good time-dependent stability and dispersion. This colloidal gold could be used to biomarkers directly. Furthermore, this method has obvious advantages over other three above-mentioned methods.

关 键 词：胶体金 生物标记 柠檬酸钠-鞣酸还原法 柠檬酸三钠还原法 抗坏血酸还原法 硼氢化钠还原法 

分 类 号：Q819[生物学—生物工程]