淹水条件下硝态氮对甜樱桃根系抗氧化酶活性和活性氧含量的影响  

Effects of NO_3^- on antioxidant enzyme activity and reactive oxygen content in roots of sweet cherry under water-logging condition

作　　者：王闯[1,3] 郭修武[2] 胡艳丽[1] 毛志泉[1,2] 陈强[1] 高相彬[1] 李静[1] 郝云红[1] 王元征[1] 张玉帅[1] 

机构地区：[1]作物生物学国家重点实验室,山东农业大学园艺科学与工程学院,泰安271018 [2]沈阳农业大学园艺学院,沈阳110161 [3]聊城职业技术学院,山东聊城252000

出　　处：《植物营养与肥料学报》2008年第1期167-172,共6页Journal of Plant Nutrition and Fertilizers

基　　金：山东省农业良种工程项目资助

摘　　要：为探讨淹水条件下NO3-对根系抗氧化酶活性和活性氧(ROS)含量的影响,以美早/Colt甜樱桃(Prunus pseudo-cerasusLindl.)为试验材料,研究了NO3-在淹水过程中对甜樱桃根系抗氧化酶活性和活性氧含量的变化。结果表明,淹水过程中甜樱桃根系超氧化物岐化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和过氧化氢(H2O2)、超氧阴离子(O2.-)、丙二醛(MDA)含量变化皆呈先升后降趋势。加入NO3-,甜樱桃根系SOD、POD和CAT活性亦呈先升后降趋势,但均高于对照;而H2O2、O2.-和MDA含量低于对照。因此,淹水条件下,加入NO3-可以提高抗氧化酶活性、降低活性氧含量,从而减轻甜樱桃根系因淹水造成的伤害。The objective of this study is to elucidate the effects of NOr on antioxidant enzyme activity and reactive oxygen content in roots under water-logging condition. We conducted a pot study using Meizao/Colt sweet cherry （ Prunus pseudocerasus Lindl. ）. They were transferred into the solution with 0, 10, 20, 30 mmol/L KNO3 and 10 mmol/L K2SO4 for 6 days to determine antioxidant enzyme activity and reactive oxygen content in sweet cherry roots. The results showed that under water-logging condition, the activity of superoxide dismutase （SOD）, peroxidase （POD）, catlase （CAT） and the contents of superoxide （Of）, hydrogen peroxide （H2O2）, and malondialdehvde （MDA） in roots of sweet cherry increased significantly in the early period of treatment and then decreased in the later period. The developmental stages of NO3^- treatments was the same as CK. NO3^- enhanced the activities of antioxidant enzymes including SOD, POD and CAT, which were higher than CK, while the concentrations of O2^-, H2O2 and MDA were lower than CK and decreased under water-logging condition. We conclude that the addition of NO3^- can alleviate the caused by water-logging condition on sweet cherry by increasing antioxidant enzyme activities, and decreasing reactive oxygen content.

关 键 词：硝态氮 淹水 甜樱桃根系 抗氧化酶 活性氧 

分 类 号：S662.5[农业科学—果树学]