大豆质核互作雄性不育系NJCMS1A和其保持系的不同器官蛋白质组比较  

Comparative Proteome Analysis of Different Organs between Cytoplasmic-nuclear Male-sterile Line NJCMS1A and Its Maintainer in Soybeans

作　　者：曾维英[1,2] 杨守萍[1] 盖钧镒[1] 喻德跃[1] 

机构地区：[1]南京农业大学大豆研究所,国家大豆改良中心,作物遗传与种质创新国家重点实验室,江苏南京210095 [2]广西农业科学院玉米研究所,广西南宁530227

出　　处：《大豆科学》2008年第1期8-14,共7页Soybean Science

基　　金：国家重点基础研究发展计划项目(2002CB111304,2004CB7206,2006CB101708);国家自然科学基金项目(30490250,30671314);国家高技术研究发展计划项目(2002AA211052,2006AA100104);长江学者和创新团队发展计划资助项目(PCSIRT)

摘　　要：质核互作雄性不育在杂种优势利用中起着重要作用,探讨质核互作雄性不育发生的机理具有重要的理论和实践意义。本研究开展大豆质核互作雄性不育系NJCMS1A与其保持系NJCMS1B的不同器官蛋白质组比较分析。以大豆质核互作雄性不育系NJCMS1A和其保持系NJCMS1B的种子、叶片和花药为材料,采用双向凝胶电泳技术对其蛋白质进行分离,考马斯亮蓝染色,获得重复性好的蛋白质双向电泳图谱,利用PDQuest软件处理分析,寻找差异表达蛋白。结果发现不育系NJCMS1A与其保持系NJCMS1B的种子2-DE图谱间存在7个差异表达蛋白点,其中3个在NJCMS1A中表达而在NJCMS1B中缺失,3个在NJCMS1A中缺失而在NJCMS1B中表达,1个在NJCMS1A中表达量明显增强;苗期叶片2-DE图谱间基本一致,没有差异表达蛋白点;单核小孢子期花药2-DE图谱间有9个差异表达蛋白点,其中3个在NJCMS1A中表达而在NJCMS1B中缺失,6个在NJCMS1A中缺失而在NJCMS1B中表达;二胞花粉期花药2-DE图谱间有24个差异表达蛋白点,其中10个在NJCMS1A中表达而在NJC-MS1B中缺失,12个在NJCMS1A中缺失而在NJCMS1B中表达,2个在NJCMS1B中表达量明显增强。两系花药间存在较多差异表达蛋白点,种子间仅有少量差异表达蛋白点,而苗期叶片间基本没有差异表达蛋白点,说明不育基因表达具有时空性和器官特异性,与育性有关的蛋白主要在花药中表达。Cytoplasmic-nuclear male sterility plays an important role in the production of hybrid seeds. The knowledge of the mechanism of the cytoplasmic-nuclear male-sterility is essential to hybrid seed production, The present paper was aimed at the comparative proteome analysis of different organs between the cytoplasmic-nuclear male-sterile line NJCMS1A and its maintainer NJCMS1B in soybeans. The materials were the seeds, young leaves and anthers from the male-sterile line NJCMS1A and its maintainer NJCMS1B. Two-dimensional gel electrophoresis （2-DE） technique was used to separate the protein spots and the gels were stained with Coomassie Blue G-250. The obtained 2-DE maps were pretty consistent among replications. The difference between the protein maps of NJCMS1A and those of NJCMS1B was analyzed with the PDQuest im- age software. Seven protein spots were found differentially expressed between NJCMS1A and NJCMS1B seeds. Among these, 3 protein spots were present in the seed protein map of NJCMS1A but absent in that of NJCMS1B,and 3 protein spots present in that of NJCMS1B but absent in that of NJCMS1A,another protein spot was up-regulated significantly in the map of NJCMS1A in comparison with that of NJCMS1B. Nine protein spots were found differentially expressed between NJCMS1A and NJCMS1B anthers at uninucleate rnicrospore stage, Among them,3 protein spots were present in the anther protein map of NJCMS1A but absent in that of NJCMS1B,and 6 protein spots present in that of NJCMS1B but absent in that of NJCMS1A. Twenty-four protein spots were found differentially expressed between NJCMS1A and NJCMS1B anthers at binucleate pollen stage. Among them, 10 protein spots were present in the anther protein map of NJCMS1A but absent in that of NJCMS1 B, and 12 protein spots present in that of NJCMS1B but absent in that of NJCMS1A,another two protein spots were upregulated significantly in the map of NJCMS1B in comparison with that of NJCMS1A. No protein spot was found differentially expressed between NJCMS1A and NJCMS1B l

关 键 词：大豆 质核互作雄性不育 不同器官 蛋白质组 双向凝胶电泳 

分 类 号：S565.1[农业科学—作物学]