借4,5-二溴苯基荧光酮螯合物和反相高效液相色谱测定铂  

Determination of Platinum as Chelate With 4, 5-Dibromophenylfluorone by Reversed-phase High-performance Liquid Chromatography

作　　者：张孝松[1] 时亮[1] 徐霁昀[1] 林长山[1] 

机构地区：[1]中国科学技术大学化学系,合肥230026

出　　处：《分析化学》1997年第4期408-411,共4页Chinese Journal of Analytical Chemistry

摘　　要：在 Ｎｕｃｌｅｏｓｉｌ　Ｃ１８柱（５μｍ，２５０×４．６ ｍｍ）上，研究了 Ｐｔ（Ⅱ）－４，５－二溴苯基荧光酮螯 合物分离和检测的最宜的流动相组成。用含０．０１ ｍｏｌ／Ｌ的ＨＡｃ－ＮａＡｃ（ｐＨ ５．０）的乙腈－水 （７０：３０，Ｖ／Ｖ）作流动相，流速为 ０．４ ｍＬ／ｍｉｎ，于 ５４０ｎｍ处检测。测定铂的校准线的浓度范 围是０．０３～０．３×１０－６ｇ／ｍＬ；检测限为０．０２０ｎｇ。方法之选择性和灵敏度甚高，已应用于实 样分析。On a Nucleosil C18 column(5 μm, 250 × 4. 6 mm i. d. ), the optimum composition of mobile phase for the separation and detection of the chelate of platinum(Ⅱ) with 4, 5-di- bromophenylfluorone was investigated. An acetonitrile-water (70: 30, V/V) solution con- taining 0. 01 mol/L acetic acid-sodium acetate buffer(pH 5. 0) was used as the mobile phase at a flow rate of 0. 4 mL/min, and the chelate was detected at 540 nm. The calibration graph was rectilinear from 0. 03-0. 3?l0-6 mg/L for platinum. The detection limit was 0. 02 ng, being the highest detection sensitivity among the reported articles for determination of plat- inum by HPLC-spectrophotometry. The proposed method has been applied to the analysis of practical samples.

关 键 词：铂 二溴苯基荧光酮 液相色谱 HPLC 

分 类 号：O614.826[理学—无机化学]