绞股蓝对金地鼠颊囊癌前病变细胞动力学影响的研究  

The study of influencing cellular dynamics of gynostemma pentaphyllum on golden hamster cheek pouch premalignancy

作　　者：周曾同[1] 徐维宁[1] 张水龙[1] 周永梅[1] 金芝贵 汪铮[1] 李伟国 张昱旻 

机构地区：[1]上海第二医科大学口腔医学院口腔内科教研室,200011

出　　处：《上海口腔医学》1997年第2期65-67,110,共4页Shanghai Journal of Stomatology

摘　　要：对癌前病变的研究是攻克癌症的重要领域之一。为了研究口腔粘膜癌前病变致癌过程的发生机制，探寻有效的防癌阻癌药物，本实验采用Salley法诱导金地鼠颊囊上皮癌变，以中草药绞股蓝（GP）行阻断试验，并以光镜结合增殖细胞核抗原（PCNA）和溴脱氧尿嘧啶（BrdU）等免疫组化方法，动态观察GP的疗效，结果发现该药能推迟白斑的出现时间和降低出现率，并能明显降低致癌率。用药组的PCNA和BrdU阳性细胞标识率明显低于模型组，且始终处于较低水平，证实了GP的防癌效果和对细胞异常增殖的抑制作用。Studying premalignancy is an importanmt field of conquering cancer. The aimto study the mechanics of oral mucosal premalignant formation and seek effective drug whichcould prevent and block cancer,Gynostemma Pentaphyllum (GP)was applies to block carcino-matous conversion of golden hamster cheek pouch by Salley's way. Using light microscopicobservation and proliferating cell nuclear antigen (PCNA) as well as bromodeoxyuridine(BrdU) (PCNA and BrdU are immunohistochemistry techniques), GPs effect was observed dy-namically. We found that GP could delay the presence of leukoplakia and decrease the rate ofpresense as well as cancer-causing rate The positive cells of taking drug group PCNA and Br-dU were lower than model group significantly and in a lower condition all the time it is illus-trated that GP has the effect of preventing cancer and inhibiting cellular dysplasis

关 键 词：绞股蓝 癌前病变 细胞动力学 口腔粘膜肿瘤 中药 

分 类 号：R286.0[医药卫生—中药学] R739.85[医药卫生—中医学]