EDC/NHS交联对胶原物理化学性能的影响  

Influence of EDC/NHS Crosslinking on Physicochemical Properties of Collagen

作　　者：王迎军[1] 杨春蓉[1] 汪凌云[1] 

机构地区：[1]华南理工大学材料科学与工程学院,广东广州510640

出　　处：《华南理工大学学报（自然科学版）》2007年第12期66-70,96,共6页Journal of South China University of Technology(Natural Science Edition)

基　　金：国家"973"重大基础研究项目(2005CB623902);广东省自然科学基金团队项目(4205786)

摘　　要：为了提高胶原的物理化学性能,采用1-乙基-3-(3-二甲基氨丙基)-碳化二亚胺(EDC)和N-羟基琥珀酰亚胺(NHS)交联剂对胶原进行交联,考察了不同交联剂浓度对胶原物理化学性能的影响,并通过红外、差示扫描量热分析(DSC)、吸水率、膨胀动力学、抗酶解性能、扫描电镜(SEM)等技术手段对胶原交联前后的性能进行表征.研究结果表明,胶原经EDC/NHS交联后,热稳定性、形态稳定性增强,抵抗酶解的能力显著增加,胶原的显微结构由交联前的无序状态变为紧密有序的结构.说明EDC/NHS交联可有效改善胶原的物理化学性能.In order to improve the physicochemical properties of collagen, 1-ethyl-3-（3-dimethyl aminopropyl） carbodiimide （EDC）/N-hydroxysuccinimide （NHS） was used as a crosslinking agent for collagen matrices, and the effect of crosslinking agent dosage on the physicochemical properties of collagen was investigated. Then, the measurements of FF-IR, DSC and SEM were performed and the water absorption, the swelling ratio and the collagenase resistance were tested to characterize the physicochemical properties of the collagen before and after the crosslinking. The results demonstrate that, after the crosslinking, the thermal stability, the morphological stability and the enzymatic resistance all improve significantly, and that the disordered microstructure of collagen changes to a dense and ordered state. It is thus concluded that the EDC/NHS crosslinking greatly improves the physicochemical properties of collagen.

关 键 词：胶原 交联 物理化学性能 1-乙基-3-(3-二甲基氨丙基)-碳化二亚胺 N-羟基琥珀酰亚胺 

分 类 号：O636.1[理学—高分子化学] TQ314.4[理学—化学]