CD151基因转移对大鼠心肌梗死缺血心肌eNOS表达影响的实验研究  

CD151 gene transfer activates eNOS in rat myocardial infarction model

作　　者：郑振中[1] 刘正湘[2] 王梦洪[1] 郑泽琪[1] 彭景添[1] 魏云锋[1] 

机构地区：[1]南昌大学第一附属医院心血管内科,330006 [2]华中科技大学同济医学院附属同济医院心血管内科,武汉430030

出　　处：《中国急救医学》2008年第2期151-154,共4页Chinese Journal of Critical Care Medicine

基　　金：国家自然科学基金资助项目(No·30570728);湖北省卫生厅科研基金重点项目(No·JX2A04)

摘　　要：目的通过将携带人CD151基因的重组腺病毒相关病毒(rAAV)载体注入缺血心肌,观察CD151对缺血心肌eNOS表达的影响。方法制作大鼠急性心肌梗死模型,将rAAV-CD151注入缺血心肌。4周后取注射部位心肌,用逆转录聚合酶链式反应(RT-PCR)检测外源性CD151mRNA的表达,Western blot检测CD151的表达,检测eNOS的表达情况,了解高表达CD151对eNOS表达的影响。结果术后4周,CD151组心肌组织检测到外源性CD151mRNA的表达,假手术组、对照组、GFP组未检测到外源性CD151mRNA的表达,且CD151组心肌组织CD151蛋白表达水平高于假手术组、对照组、GFP组(P<0·05);CD151组eNOS与磷酸化eNOS比值明显增加,与对照组、GFP组比较明显增加(P<0·05)。结论rAAV-CD151可以有效地转染大鼠心肌组织,激活eNOS,促进缺血心肌的血管生成,改善左室功能。Objective To investigate the effect of CD151 gene divery on eNOS in rat myocardial infarction model. Methods Rat myocardial infarction model was formed by ligation of the anterior descending coronary artery, and rAAV - CD151 was injected into the ischemic myocardium. At 4 weeks after coronary artery ligation, human CD151 mRNA was detected using RT - PCR, Western blot analysis for CD151,phosphor-eNOS and total eNOS were performed. Results We found that human CD151 mRNA was detected in rAAV- CD151 group. Compared with the rAAV- GFP group and controll group,CD151 gene delivery significantly increased the expression of CD151 （P 〈 0. 05） and the level of PI3K, the ratio of phosphor - eNOS/total eNOS after myocardial infarction in ischemic myocardium （ P 〈 0. 05 ）. Conclusion This study suggested that CD151 could activate eNOS after myocardial infarction in rats.

关 键 词：CD151 心肌梗死 一氧化氮合酶 

分 类 号：R542.22[医药卫生—心血管疾病]