流动注射-化学发光法测定片剂、人血清及尿液中6-巯基嘌呤的含量  

A flow injection chemiluminescence method for determination of 6-mercaptopurine in its tablets, human serum and urine

作　　者：孙汉文[1] 苏明[1] 李丽清[1,2] 陈雪艳[1] 

机构地区：[1]河北大学化学与环境科学学院河北省分析科学技术重点实验室,保定071002 [2]泰山学院化学系,泰安271021

出　　处：《药物分析杂志》2008年第2期219-222,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：教育部博士点学科基金项目(No.20050075003)

摘　　要：目的:研究6-巯基嘌呤-高锰酸钾-甲醛-多聚磷酸体系的化学发光行为与机理,建立流动注射-化学发光法测定片剂、人血清与尿液中6-巯基嘌呤的含量。方法:采用6-巯基嘌呤-高锰酸钾-甲醛-多聚磷酸化学发光体系,基于化学发光强度与浓度间的线性关系对6-巯基嘌呤加以定量测定。结果:校准曲线的线性范围为1.0×10^(-8)～1.0×10^(-5)g·mL^(-1),检出限为6×10^(-9)g·mL^(-1);对2.0×10^(-7)g·mL^(-1)6-巯基嘌呤进行11次平行测定,RSD 为1.7%;片剂、人血清和尿液加样回收率在95.0%～111.3%范围内,RSD 为1.8%～2.2%。结论:本方法简便、灵敏、可靠,可用于6-巯基嘌呤的常规检测及药代动力学研究。Objective ：To study chemiluminescence characteristic and mechanism of the potassium permanganate - formaldehyde- 6 -mercaptopurine polyphosphate system and to establish a novel method for determination of 6 - mercaptopurine in pharmaceutical preparations ,human serum and urine by flow injection chemiluminescence techniqure. Methods： Potassium permanganate - formaldehyde 6 - mercaptopurine polyphosphate was adopted in chemi- luminescence system. 6 - mercaptopurine was quantitatived based on linear relationship between the chemiluminescence intensity and the concentration. Results：The linear range of the calibration curve was 1.0 ×10^-8 - 1.0× 10^-5 g · mL^-1 ,and the detection limit was 6 × 10^-9 g · mL^-1. The RSD was 1.7% for 11 measurements at 2. 0 × 10^-7 g · mL^-1 6 -mercaptopurine standard solution. The recoveries for spiked pharmaceutical preparations, serum and urine were in the range of 95.0% - 111.3%. The results agree well with those obtained by the Pharmacopoeia standard method. Conclusion：The proposed method is simple, sensitive and reliable. It can be applied for routine determination of 6- mercaptopurine and pharmacokinetic study.

关 键 词：流动注射 化学发光 6-巯基嘌呤 药剂 血清 尿液 

分 类 号：R917[医药卫生—药物分析学]