流动注射纳米微反应器化学发光法测定盐酸曲马多  

Flow-injection chemiluminescence with nanometer microreactor analysis of tramadol hydrochloride

作　　者：石文兵[1] 杨季冬[1] 江虹[1] 徐建华[1] 

机构地区：[1]长江师范学院化学系,涪陵408003

出　　处：《药物分析杂志》2008年第2期235-238,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：重庆市教委资助项目(No.KJ061306);重庆市科技攻关计划项目;发光与实时分析重庆市重点实验室开放基金项目;长江师范学院资助项目

摘　　要：目的:建立盐酸曲马多的化学发光测定新方法。方法:在酸性条件下,盐酸曲马多分子中氮原子被质子化后与阴离子AuCl_4^-形成离子缔合物,该缔合物被二氯甲烷带入鲁米诺的氯化十六烷基三甲基铵反胶束纳米微反应器中,离解出来的AuCl_4^-立即与鲁米诺产生化学发光。在一定浓度范围内,发光强度与盐酸曲马多的含量呈线性关系,从而间接测定盐酸曲马多的含量。结果:在优化的试验条件下,线性范围为0.001～30μg·mL^(-1),检出限(3σ)为0.02 ng·mL^(-1),对浓度为1.0μg·mL^(-1)的盐酸曲马多进行11次平行测定,RSD 为1.3%。结论:该法已成功用于片剂、注射液和生物体液中盐酸曲马多的测定。Objective：To establish a flow injection chemiluminescence （CL） method for the determination of tramadol hydrochloride. Methods：In acidic condition, the nitrogen in tramadol hydrochloride was easily protonated forming ion - complex with negative ion AuC14 , the ion - complex was extacted and carried by dichloromethane, when the ion - complex entered a reversed micellar nanometer microreactor of cetyltrimethylammonium chloride containing luminol,the dissociated AuCl4ˉ reacted with luminol and produced analytical chemiluminescence. Results：In the optimum conditions,the linear range was 0. 001 to 30 μg · mL^-1 and the limit of detection was 0. 02 ng · mL^-1 with a relative standard deviation （n = 11 ） of 1.3% for 1.0 μg · mL^-1 tramadol hydrochloride. Conclusion：The method has been applied to the determination of the studied drug in tablets, injections and biological fliuds successfully.

关 键 词：纳米微反应器 盐酸曲马多 化学发光 

分 类 号：R917[医药卫生—药物分析学]