高效毛细管电泳法测定磺胺类药物的离解常数及其制剂的含量  

HPCE determination of dissociation constants of sulfonamides and their contents

作　　者：李玉琴[1] 张传港[1] 宗素艳[2] 崔英杰[3] 贾宝秀[1] 赵晓明[3] 曹明亮[1] 齐永秀[1] 

机构地区：[1]泰山医学院药学院,泰安271016 [2]南京工业大学制药与生命科学学院,南京210009 [3]泰山医学院生命科学研究所,泰安271016

出　　处：《药物分析杂志》2008年第2期243-246,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：泰山医学院博士启动基金(2006-742)

摘　　要：目的:采用毛细管电泳法测定磺胺甲唑(sMZ)、磺胺嘧啶(SD)和甲氧苄啶(TMP)的离解常数及其在制剂中的含量。方法:采用未涂层的弹性石英毛细管柱(60.2 cm×75 μm,有效长度50 cm),柱温25 ℃,正极压力进样,检测波长214 nm。将3种化合物的有效电泳淌度与 pH 进行非线性拟合,测定其离解常数;最佳的分离条件为:50 mmol·L^(-1)磷酸二氢钠(pH 6.0)缓冲液,分离电压27.5 kV,在此条件下测定其在制剂中的含量。结果:SMZ、SD、TMP 的离解常数分别为5.69,6.36,6.68;在0.1～1000μg·mL^(-1)的浓度范围内线性关系良好(相关系数:0.9992～0.9999),回收率为95.6%～105.0%。结论:该方法简便、快速、准确,可用于测定3种磺胺类药物的解离常数及其在制剂中的含量。Objective：To develop a simultaneous determination the dissociation constant values of sulfamethoxazole （SMZ） ,sulfadiazine（SD） and trimethoprim（ TMP）, and the contents in their preparations by HPCE. Method： A fused silica capillary column （60.2 cm× 75 μm, effective length 50 cm） was used, the cartridge temperature was 25 ℃, sample was introduced from the anode with pressure injection ,the UV detection wavelength was 214 nm. The dependence of effective mobility of the three compound on pH was investigated, and their apparent dissociation constant values determined by nonlinear simulation;the best separation was achieved with a running buffer of 50 mmol · L^-1 NaH2PO4（pH 6.0） and at the applied voltage of 27.5 kV for determination the contents of the three compounds. Result： The dissociation constant values （ pKa ） of SMZ, SD and TMP were 5.69,6.36 and 6.68, respectively. The linear concentration range of the three compounds was 0. 1 - 1000 μg · mL^-1 with a correlation coefficient of 0. 9992 -0. 9999. The recoveries of the three analytes were 95.6% - 105.0%. Conclusion：The proposed method is simple, fast and accurate for determination the dissociation constant values of sulfonamides and their contents.

关 键 词：高效毛细管电泳 磺胺甲噁唑(SMZ) 磺胺嘧啶(SD) 甲氧苄啶(TMP) pK 

分 类 号：R917[医药卫生—药物分析学]