RP-HPLC同时测定菝葜药材中总槲皮素和山柰酚含量  

RP-HPLC simultaneous determination of quercetin and kaempferol in Smilax china L.

作　　者：颜彦[1] 李文霞[2] 叶晓川[1] 

机构地区：[1]华中科技大学生命科学与技术学院药物研究所,武汉430074 [2]华中科技大学化学系,武汉430074

出　　处：《药物分析杂志》2008年第2期288-290,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立同时测定菝葜药材中槲皮素和山柰酚含量的反相高效液相色谱法。方法:色谱柱:Lichrospher C_(18)柱(250 mm×4.6 mm,5 μm),流动相:甲醇-0.5%磷酸水溶液(55:45),流速:1.0 mL·min^(-1),检测波长:365 nm。结果:槲皮素在2.97～29.7μg·mL^(-1)浓度范围内线性关系良好(r=0.9999),山柰酚在3.01—30.1μg·mL^(-1)浓度范围内线性关系良好(r=0.9999),方法回收率分别为98.9%和99.7%。结论:本方法简便、准确,灵敏度高,重复性好,可为评价菝葜药材质量提供依据。Objective ： To establish an RP - HPLC method for the determination of quercetin and kaempferol content in Smilax china L. Methods：The C18 column（250 mm ×4. 6 mm,5 μm） was used for analysis,methanol -0.5% H3PO4 （55：45 ） as mobile phase with detection wavelength 365 nm, 1.0 mL · min^-1 of flow rate and column temperature at room temperature. Results： Quercetin and kaempferol showed a good linear curve in the range of 2.97 - 29.7 μg · mL^-1 （r =0. 9999） and 3.01 -30. 1μg · mL^-1 （r =0. 9999） ,respectively. The recoveries of quercetin and kaempferol were 98.9% and 99.7%. Conclusion ：This simple and reproducible HPLC analysis provided reference to estimate the quality of Smilax china L. The analytical results were reliable and accurate.

关 键 词：菝葜 槲皮素 山柰酚 RP—HPLC 

分 类 号：R917[医药卫生—药物分析学]