液相色谱-串联四极杆质谱联用法测定中药降糖制剂中掺入罗格列酮的研究  

Detection of rosiglitazone added in traditional Chinese medicinal antidiabetic preparations by the liquid chromatography-quadrupole mass spectrometry method

作　　者：邱颖姮[1] 王铁杰[1] 李玉兰[1] 庞学斌[1] 王玉[1] 李军[1] 

机构地区：[1]深圳市药品检验所,深圳518029

出　　处：《药物分析杂志》2008年第2期323-325,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立检测中药降糖制剂中非法掺入的罗格列酮专属性方法。方法:采用液相色谱-串联四极杆质谱联用法。通过相对分子质量、二级质谱碎片信息、液相色谱保留时间和紫外光谱四方面信息,对中药降糖制剂的提取液进行液相色谱-串联四极杆质谱分析。通过与对照品的光谱、色谱及质谱行为相比较,对中药降糖制剂中非法掺入的合成降糖药进行定性鉴别。结果:在4种受试中药降糖制剂中,2种被检测到掺有罗格列酮。结论:该方法选择性强,灵敏度高,可作为分析检测中药降糖制剂中非法掺入罗格列酮的比较有效的方法。Objective：To develop a specific method for the identification of rosiglitazone added in traditional Chinese medicine antidiabetic preparations and test some formulations. Methods： The liquid chromatography - quadrupole mass spectrometry method was used. After separated by UV, HPLC, the organic extraction of the traditional Chinese medicine antidiabetic preparations was analyzed. The compounds illegally added into the traditional Chinese medicine antidiabetic preparations were identified according to the spectrum, chromatographic behavior and mass spectral data by comparison with those of reference substances. Results： Rosiglitazone was found in two formulations. Conclusion：The method is selective and sensitive and can be used to detect the illegal traditional Chinese medicine antidiabetic preparations.

关 键 词：中药降糖制剂 罗格列酮 液相色谱-串联四极杆质谱法 

分 类 号：R917[医药卫生—药物分析学]