有氧训练对大鼠局灶性脑缺血后神经功能修复的实验研究  

Impact of Aerobic Exercise on Repair of Nerve Function after Middle Cerebral Artery Occlusion in Rat

作　　者：吴伟奋[1] 陈春美[2] 董华[1] 孙镭[1] 杨卫忠[2] 

机构地区：[1]福建医科大学体育教研部,福建福州350004 [2]福建医科大学协和临床医学院神经外科,福建福州350001

出　　处：《体育科学》2008年第2期59-62,共4页China Sport Science

基　　金：福建省教育厅科技计划项目(JA04200)。

摘　　要：目的:研究有氧运动对大鼠局灶性脑缺血后神经功能修复和促进eNOS表达的机制。方法:建立局灶性脑缺血SD大鼠模型后,运动组采用适度游泳训练,对照组制动。分析大鼠神经功能缺失评分和缺血神经细胞超微结构;检测缺血区域一氧化氮(NO)的含量、一氧化氮合酶(NOS)的活性和内皮细胞型NOS(eNOS)基因表达量。结果:电镜观察对照组缺血神经细胞溶解,空泡变性,运动组细胞形态结构和细胞器完整;比较缺血10d的对照组和运动组,神经功能缺失评分、NO含量、NOS的活性和eNOS mRNA表达量之间的盖异无统计学意义(P>0.05);而在缺血30 d和60 d的对照组和运动组之间,神经功能缺失评分、NO含量、NOS的活性和eNOS mRNA表达量之间的差异有统计学意义(P<0.05)。结论:有氧训练能够增强局部脑缺血区域神经细胞eNOS基因的表达,提高NO的合成量,改善局部缺血区域血运,促进损失神经细胞的恢复。The purpose of this study was to investigate the impact of aerobic exercise on repair of nerve function after middle cerebral artery occlusion in rat. The exercise group was trained with swimming, while the control group was not after a rat model of cerebral ischemia was established. The score of nerve function in different group was calculated, and the ultrastructure of two groups was observed. Both content of NO and activity of NOS in different group were compared. The expression of eNOS gene in different group was analyzed by RT-PCR. The control group cell showed cell disruption and vacuolus, the exercise group showed cellular structure integrity under the observation of transmission electromicroscope. There was no difference in score of nerve function, content of NO, activity of NOS and expression of eNOS gene between two groups with isehemic 10 days, but significant difference in those between two groups with ischemic 30 days and 60 days. It concludes that the aerobic exercise can enhance the expression of eNOS gene, elevate the amount of NO, and increase the blood fluency on middle cerebral artery occlusion brian and improve the nerve function.

关 键 词：有氧运动 脑缺血 神经功能 修复 内皮细胞型一氧化氮合醇 鼠 动物实验 

分 类 号：G804.7[文化科学—运动人体科学]