扇贝裙边糖胺聚糖体外抗I型单纯疱疹病毒实验研究  

Experimental study of the antiviral effect of glycosaminoglycan from scallop skirt on herpes simplex virus type Ⅰ in vitro

作　　者：于囡[1] 刘赛[1] 王海桃[1] 高艳丽[1] 

机构地区：[1]青岛大学医学院药理学教研室,山东青岛266021

出　　处：《中国药理学通报》2008年第2期210-213,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(NoA30171157);山东省中医药科技发展计划资助项目(No2005-016)

摘　　要：目的研究扇贝裙边糖胺聚糖（glycosaminoglycan from Scallop Skirt，SS-GAG）体外抗单纯疱疹病毒Ⅰ型（HSV-Ⅰ）的作用。方法运用单纯疱疹病毒Ⅰ型（HSV-ⅠSM44株），并以非洲绿猴肾细胞（Vero细胞）为宿主细胞，通过观察病毒感染后的细胞变性反应（cytopathiceffect，CPE）和运用MTF比色法，检测不同浓度药物SS—GAG对Ⅰ型单纯疱疹病毒是否有直接的灭活作用、对HSV—Ⅰ感染复制的抑制活性以及对HSV—Ⅰ感染细胞的综合作用等，并观察药物对Vero细胞的毒性作用。结果与病毒对照组相比，SS-GAG各浓度组（100、50、25mg·L^-1）能有效地保护经HSV—Ⅰ感染的Vero细胞，使细胞活性增强（P〈0．01）；并减弱HSV—Ⅰ导致的病变效应，抑制病毒的复制。此作用随着药物浓度的增加而增强。但是SS—GAG对HSV—Ⅰ没有直接的灭活作用；SSGAG在50～1600mg·L^-1浓度范围内对Vero细胞无明显的细胞毒性。结论SS-GAG在体外实验系统中显示出明显的保护宿主细胞抵抗HSV-Ⅰ病毒感染的活性作用。Aim To study the antiviral effects of glycosaminoglycan from Scallop Skirt （SS-GAG） on herpes simplex virus type Ⅰ （ HSV-Ⅰ ） in vitro. Methods The direct effect on virus, the influences of SS-GAG on virus reproduction and synthesis effect in virus infecting cells were investigated in vitro utilizing the herpes simplex virus type I and taking vero cell as host cell by MTT colorimetry and observing cytopathic effect. The cytoxicity of SS-GAG was measured by MTT colorimetry. Results Compared with the virus control group, SS-GAG-treated group could protect the vero cells effectively and heighten the cell activity（ P 〈0. 01 ）. SS-GAG-treated group could lessen the cytopathic effect induced by HSV-Ⅰ and inhibit virus replication. These effects became stronger with the increase of concentrations of SS-GAG. But SS-GAG was not observed to have a direct inhibitory effect on the virus. The SS- GAG did not show cell toxicity on vero cells within the concentrations of 50 - 1600 mg ·L^-1. Conclusion SS-GAG showed siginificant activity of protecting host cells and anti-viral effect on HSV-Ⅰ in vitro.

关 键 词：扇贝裙边 糖胺聚糖 单纯疱疹病毒Ⅰ型 抗病毒作用 

分 类 号：R-332[医药卫生] R282.74