沙樱桃黄酮对人A-704细胞毒性及增殖的影响  

Cytotoxic and proliferous effects of Nitraria tangutorum flavone on human A-704 strain cells

作　　者：俞发荣[1] 连世军 连秀珍[1] 

机构地区：[1]甘肃政法学院公安分院实验中心,甘肃兰州730070 [2]甘肃省民勤县人民医院泌尿外科,甘肃民勤733300

出　　处：《中国药理学通报》2008年第2期236-239,共4页Chinese Pharmacological Bulletin

基　　金：甘肃政法学院“135人才工程”基金资助项目(NoGZF04-75)

摘　　要：目的探讨沙樱桃黄酮对人A-704(肾癌)细胞毒性作用及其对细胞增殖的影响,为沙樱桃的应用和开发提供实验依据。方法体外培养人A-704细胞沙樱桃黄酮,用台盼蓝法检测对细胞毒性作用;用克隆形成法测定对细胞分裂增殖的影响;用X射线能量色散谱仪分析对细胞内微量元素含量的影响。结果①台盼蓝法测定结果显示,给药后培养6h,沙樱桃黄酮(0.1,0.5,5mg.L-1)组和5-氟尿嘧啶(5-FU)组活细胞数比对照组分别减少了33.8%、35.8%、60.8%和40.3%,到18h减少了57.3%、80.1%、99%和59.6%,差异有显著性(P<0.01)。沙樱桃黄酮组间比较,差异有显著性(P<0.01)。②克隆形成法测定结果表明,沙樱桃黄酮(0.1,0.5,5mg.L-1)组和5-FU组克隆数比对照组减少了40.4%、47.6%、83.6%和43.4%,差异有显著性(P<0.01)。③X射线能量色散谱仪分析结果表明,细胞内Ca2+离子水平随给药浓度的增加而升高,K+和P3+离子浓度则降低,与对照组比,差异有显著性(P<0.01)。结论沙樱桃黄酮对人A-704细胞具有明显的毒性作用,其作用与抑制细胞分裂,改变细胞膜结构,使细胞内游离Ca2+增加活化细胞内核酸内切酶,引起细胞DNA损伤有关。Aim To observe the cytotoxic and proliferous effects of Nitraria tangutorum flavone（NTF） on human A-704 strain cells. Methods In vitro cultured human A-704 strain cells, the cytotoxic and proliferous effects of NTF on human A-704 strain cells were determined with methods of trypan-blue and clone formation, and the microelement in cell was detected with Xelectron microscope scanning. Results （1） The trypanblue detection showed that the living cells in the NTF group（0. 1,0. 5 and 5 mg ·L^-1 ）and the 5-FU group were decreased by 33.8% - 57.3%, 35.8% - 80. 1%, 60. 8% -99% and 40. 3% -59, 6% at 6 - 18 hour after treatment, which significantly differed from those of the control group（P 〈0. 01 ） ; there were signit%ant differences between the NTF groups （ p 〈 0. 01 ）. （2） The clone formation results showed that the clones in NTF group （0. 1,0. 5 and 5 mg · L^- 1 ） and the 5-FU group were decreased by 40. 4%, 47.6%, 83.6% and 43.4%, compared to the control group （ P 〈 0. 01 ）. （3） X-electron microscope scanning showed that the Ca2＋ was increasing and the K＋ , p3＋ was decreasing in the cell with the increase of NTF. Conclusion NTF has significant cytotoxic effects on human A-704 strain cells and the mechanism may be related to inhibition of cell division, destruction of structure of cell membrane and change of level of microelement.

关 键 词：沙樱桃黄酮 人A-704细胞 细胞毒性作用 微量元素 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]