人脐带和骨髓源间充质干细胞生物学特征的对比研究  

Comparative Characterization of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and Bone Marrow

作　　者：吕璐璐[1] 宋永平[1] 魏旭东[1] 房伯俊[1] 张艳莉[1] 李玉富[1] 

机构地区：[1]河南省肿瘤医院血液科,河南省血液病研究所,河南郑州450008

出　　处：《中国实验血液学杂志》2008年第1期140-146,共7页Journal of Experimental Hematology

摘　　要：为了对比人脐带组织源间充质干细胞(UC-MSC)与骨髓源间充质干细胞(BM-MSC)的生物学特性,从足月胎儿脐带组织和成人骨髓中分离出间充质干细胞(MSC)。用有限稀释法、流式细胞术、倒置显微镜检及RT-PCR等方法检测UC-MSC和BM-MSC的分离成功率、细胞产量、成纤维细胞集落形成单位(CFU-F)、增殖特性、免疫表型和多向诱导分化能力等并对比性研究二者的生物学特性。结果表明,UC-MSC和BM-MSC的分离成功率均达100%;虽然由脐带组织中分离的有核细胞数低于骨髓(1×106/cmvs5.5×107/ml)(p=0.0002),但在培养第14天由脐带和骨髓中得到的贴壁细胞数无差异(8.6×105/cmvs8.4×105/ml)(p>0.05);UC-MSC形态、大多数分子表型、细胞周期状态、脂肪和骨诱导分化能力与BM-MSC相似,但UC-MSC的CFU-F比例(1∶1609±0.18)高于BM-MSC的CFU-F比例(1∶35700±0.01)(p<0.05)。此外,UC-MSC具有比BM-MSC更强的增殖能力(p<0.05)。UC-MSCHLA-ABC和CD106分子表达低于BM-MSC(p<0.05)。结论:脐带组织源间充质干细胞产量和绝大多数生物学特征与BM-MSC的相似,且具有比BM-MSC更高的增殖能力、较低的HLA-ABC和HLA-DR表达,UC-MSC有望成为BM-MSC理想的替代来源。This study was purposed to compare the biological characteristics of umbilical cord-derived mesenchymal stem cells （UC-MSCs） and bone marrow-derived mesenchymal stem cells （BM-MSCs）. The frequency of successful isolation, cell yield, colony-forming units-fibroblastics （CFU-F）, proliferation capacity, immunophenotype and multidifferentiation potentials of UC-MSCs and BM-MSCs were determened by limiting dilution assay, flow cytometry, invert microscopy, RT-PCR and so on, the determined results were compared. The results showed that MSCs were successfully isolated from all the 36 portion of UC tissue and 8 portion of BM. Although the mean number of nucleated cells isolated from UC tissue was significantly lower than that from BMs （ 1 × 10^6/cm vs 5.5 × 10^7/ml） （p =0.0002）, no significant differences of the yield of adherent cells were observed （8.6 × 10^5/cm vs 8.4 × 10^5/ml ） （p 〉 0.05 ）. UC-MSCs shared the most of the characteristic of BM-MSC, including fibroblastic-like morphology, typical immunophenotype, cell cycle status, adipogenic and osteogenic differentiation potentials. However, the CFU-F frequency was higher in UC （ 1 ： 1609 ± 0.18） than that in BM （1：35700 ±0.01） （p 〈0.05）. Furthermore, the proliferation capacity of UC-MSCs was higher than that of BM-MSCs; the expressions of CD106 and HLA-class I in UC-MSCs were lower than those in BM-MSCs （p 〈 0.05）. It is concluded that the cell yield and most biological characteristics of UC-MSCs are similar to BM-MSCs, but UC-MSCs possess the higher proliferation capacity, and the lower expression of HLA-class I and HLA-DR as compared with BM-MSCs, therefore the human umbilical cord tissue may be considered as a promising alternative to bone marrow as a source of MSCs.

关 键 词：间充质干细胞 脐带源间充质干细胞 骨髓源间充质干细胞 生物学特征 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]