细菌筛检在预防和控制血小板细菌污染方面的有效性  

Effectiveness of Bacterial Screening in Preventing and Controlling Platelet Bacterial Contamination

作　　者：林俊杰[1] 徐忠[1] 陈敏[2] 邱颖婕[1] 张晰[1] 孔祥荣[1] 周筱嫣[1] 马庆[1] 钱开诚[1] 

机构地区：[1]上海市血液中心,上海200051 [2]上海市疾病预防控制中心,上海200051

出　　处：《中国实验血液学杂志》2008年第1期189-191,共3页Journal of Experimental Hematology

摘　　要：本研究探讨用血小板细菌筛检及24小时锁定的方法预防和控制血小板细菌污染的有效性。用BacT/A-LERT细菌培养仪对保存24小时后(22℃振荡保存)的单采血小板(机器采集的血小板)进行细菌筛检。在无菌条件下抽取5袋样品,并合并成1袋后分别接种于需氧培养液和厌氧培养液中,同时将被抽检的血小板进行锁定。接种后的培养液置培养箱中培养24小时后,如结果为阴性,则该血小板可放行。如出现阳性,对阳性结果进行菌种鉴定,并取留样样品进行复试。结果表明:8017袋单采血小板中,初筛为阳性的16袋(0.2%),复筛确认阳性的4袋(0.05%);进一步菌种分析显示3袋为金黄色葡萄球菌,1袋为耳状葡萄球菌。结论:血小板细菌筛检、24小时锁定作为血小板常规检测项目是非常必要的,它对于预防和控制血小板细菌污染是有效而且适宜的。This study was purposed to investigate the effectiveness of bacterial screening with 24 hours holding in preventing and controlling bacterial contamination of platelets. Bacterial screening of apheresis platelets preserved for 24 hours was perfomed by using BacT/ALERT automatic bacterial culture system. The samples from 5 bags of platelet were taken in aseptic condition and were merged into 1 bag. The final sample was inoculated into aerobic and anaerobic bottle respectively for testing, meanwhile the screened platelet samples were held for 24 hours. If the platelets were cultured for 24 hours and identication of bacterial strains showed negative, the platelets could be released, and the original platelet samples should be rescreened if initiate positive was found. The results showed that in screening 8017 samples of apheresis platelets the initiate positive results were 16 （0.2%） and confirmed positive were 4 （0.05%）. Out of 4 confLrmed positive strains, three were staphylococcus aureus and another was staphylococcus auricularis. It is concluded that it is necessary for blood center to apply the method of bacterial screening of platelet with 24 hours holding as conventional screening method, which is an effective and feasible way to prevent and control bacterial contamination of platelets.

关 键 词：血小板 细菌筛检 24小时锁定 血小板细菌污染 

分 类 号：R331.143[医药卫生—人体生理学]