去炎松-A固体脂质纳米颗粒透皮吸收在瘢痕治疗中的效应  

Triamcinolone acetonide acetate-loaded solid lipid nanoparticles of transdermal drug delivery in treatment of scars

作　　者：郭庆民[1,2] 薛晓东[1] 吴建[1,2] 蒋丽媛[1,2] 樊芙蓉[3] 

机构地区：[1]甘肃省人民医院烧伤整形科 [2]兰州大学,甘肃省兰州市730000 [3]兰州大学

出　　处：《中国组织工程研究与临床康复》2008年第1期35-38,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:为克服目前瘢痕非手术治疗中糖皮质激素治疗的副作用,尝试应用去炎松-A固体脂质纳米颗粒经透皮吸收技术治疗瘢痕,观察其效果。方法:实验于2007-04/08在解放军兰州军区总医院动物实验中心实验室进行。①造模:选用新西兰白兔16只,每个兔耳上将全层皮肤切除,形成1cm×1cm上、下两个创面,术后30d上皮化生逐渐形成病理性瘢痕。②分组干预:造模成功后随机分为对照组和实验组,每组8只。对照组不干预,实验组于术后30d起应用含有去炎松-A1mg/cm2的固体脂质纳米颗粒卡波姆凝胶覆于已形成的瘢痕上透皮吸收。③观察指标:分别于模型形成后第30,60,90和120天时依次取每只大白兔左耳上、下,右耳上、下的病理性瘢痕作为标本来源进行组织病理学观察,同时采用免疫组化检测组织中的ki-67表达和羟脯氨酸含量,并抽静脉血测血中皮质醇浓度。结果:16只白兔均进入结果分析。①实验组做模型后第30天时ki-67表达及羟脯氨酸含量与对照组比较无明显差异(P>0.05),但第60,90和120天实验组瘢痕组织中表达及羟脯氨酸含量较对照组明显减少(P<0.01)。②实验组及对照组各时段血中的皮质醇浓度比较无明显差异(P>0.05)。③实验组造模后120d病理性瘢痕中成纤维细胞的数量少于对照组。结论:含有去炎松-A1mg/cm2的固体脂质纳米颗粒卡波姆凝胶透皮吸收对瘢痕有治疗作用,且长期应用血液中的皮质醇浓度不升高,能预防糖皮质激素治疗瘢痕的副作用。AIM： In order to conquer the side-effects of glucocorticoid in non-operative treatment of the pathological scar, this study investigated the therapeutic effect of triamcinolone acetonide acetate （TAA）-loaded solid lipid nanoparticles （SLN） of transdermal, drug delivery for treating the scat. METHODS： From April to August in 2007, the experinaent was carried out m the Animal Central Laboratory of the General Hospital of Lanzhou Military Area Command of Chinese PLA（1）Sixtecn New Zealand white rabbits, subjected to cutting the full skin on the ears and Ibrmed two 1 × 1 cm^2 injures, were used in the study, then the pathological scar model was gradually conducted after cpithelization at 30 days postoperatively.（2）The successful models were randomly divided into control （C） group and treatment （T） group, each containing 8 animals. No treatment was given in C group after modeling, while T group was treated with transdenmal drug delivery of TAA-loaded SLN （1 mg/cm^2） carbomer gel for 90 days since the 30^th day after modeling.（3）The cutaneous tissue samples from rabbit ear scar in each group were harvested on the 30^th, 60^th, 90^th and 120^th days after modeling for the pathomorphological examination. Hydroxyproline （Hyp） content and ki-67 exprcssion were determined by immunohistochemical technique. Meanwhile the quantity of corticosteroid in venous blood was also detected. RESULTS： All the 16 rabbits were involved in the rest, It analysis.（1）Content of the Hyp and the expression of ki-67 were no remarkably significant between C group and T group （P 〉 0.05）, but higher in thc scar of rabbits in C group on the 60^th, 90^th, 120^th days after modeling compared with T group （P 〈 0.01 ）.（2）There was no significant difference in the quantity of corticosteroid between C group and T group （P 〉 0.05 ）（3）The fibroblasts in the pathological scar was reduced in T group than in C group at 120 days postoperatively. CONCLUSION： Treatment with transdermal drug

关 键 词：固体脂质纳米颗粒 病理性瘢痕 去炎松-A 透皮吸收技术 卡波姆凝胶 KI-67 羟脯氨酸 皮肤组织构建 

分 类 号：R318.08[医药卫生—生物医学工程]