聚乙烯醇-胶原凝胶研制及作为组织替代材料的生物相容性[英文]  

Development of polyvinyl alcohol-collagen composite and its biocompatibility as tissue substitute

作　　者：叶春婷[1,2] 黄耀熊[2] 邹海燕 陈鸿辉 彭燕豪 

机构地区：[1]暨南大学医学院附属广州市红十字会医院,广州市创伤外科研究所,广东省广州市510220 [2]暨南大学生物医学工程系,生物医学工程研究所,广东省广州市510632

出　　处：《中国组织工程研究与临床康复》2008年第1期153-156,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广州市科技攻关项目(200623-E0371)~~

摘　　要：背景：聚乙烯醇对细胞黏附能力有限,在聚乙烯醇中加入胶原蛋白能否改善其细胞的黏附性？目的：研制一种新型的聚乙烯醇-胶原复合材料,并探讨其作为软组织替代材料的可行性。设计：单一样本观察。单位：暨南大学医学院附属广州市红十字会医院,广州市创伤外科研究所。材料：选用15只新西兰大白兔,体质量2.0~3.0kg,雌雄不拘,由广东省医学实验动物中心提供。实验过程中对动物的处置符合动物伦理学标准。广州市创伤外科研究所市级实验室完成。牛I型胶原购自广州创尔生物技术有限公司,聚乙烯醇-124购自广州南方化玻仪器公司。方法：实验于2003-07/2006-12在暨南大学医学院附属广州市红十字会医院完成。①聚乙烯醇-胶原材料制备：配制5g/L的牛Ⅰ型胶原与5%的聚乙烯醇-124以1∶1混合,真空冷冻干燥成为凝胶状,扫描电镜观察其内部结构。②细胞毒性试验：聚乙烯醇-胶原材料剪成10mm×5mm×1mm小片,γ射线消毒后,分别放入48孔平板中,每孔加入1×104个3T3细胞培养,扫描电镜观察细胞的生长状况。③体内埋植试验：将实验兔背部脊柱两侧各作2个纵切口,形成皮下腔隙。分别于手术形成的皮下腔隙植入4块聚乙烯醇-胶原材料,分别于术后1,4,8周各取3只实验共9块标本及其周围组织作病理观察。主要观察指标：扫描电镜观察凝胶膜内部结构、细胞毒性试验及体内埋植试验结果。结果：①聚乙烯醇-胶原材料内部结构：真空冷冻干燥的聚乙烯醇-胶原为白色的凝胶,扫描电镜见其表面和切面内部有贯通的三维孔隙。②细胞毒性试验结果：3T3细胞在PVA-胶原材料上能三维生长、形态正常。③体内埋植试验结果：聚乙烯醇-胶原植入1周后,产生了正常的异物反应,材料与组织界限分明。4周后,只见极少量淋巴细胞浸润,大量的纤维母细胞增生形成胶原纤维和假单层立�BACKGROUND： Polyvinyl alcohol （PVA） displays limitation to cell adsorbability. Can collagen improve the adsorbability of PVA to cells？ OBJECTIVE： To develop a novel type composite of PVA and collagen, and explore the feasibility to serve as soft tissue substitute. DESIGN： Single sample observation. SETTING： Guangzhou Red Cross Hospital, Jinan University Medical College, Guangzhou Institute of Traumatic Surgery. MATERIALS： Fifteen New Zealand rabbits of 2.0-3.0 kg, either male or female, were provided by Medical Experimental Animal Center of Guangdong Province. The experiment was carried out in the Laboratory of Guangzhou Institute of Traumatic Surgery, and the experimental procedure was accorded with the animal ethical standards. Bovine type I collagen was purchased from Guangzhou Trauer Biotechnology Co., Ltd. and PVA-124 from Guangzhou Chemical Reagent and Instrumentation Co., Ltd. METHODS： The experiment was performed in Guangzhou Red Cross Hospital, Jinan University Medical College between July 2003 and December 2006. （1）Preparation of PVA-collagen material： 5 g/L bovine type I collagen was mixed with 5% PVA-124 at a ratio of 1 ： 1. The mixture was freeze-dried at vacuum until becoming gelatinous. The internal structure was observed under the use of scanning electron microscope. （2）Cytotoxicity test： PVA-collagen composite was cut into pieces of 10 min×5 min×1 mm, put into 48-well culture plate after sterilized by Y ray, cultured with 1× 10^4 3T3 cells in each well. Cell growth was observed under scanning electron microscope and laser scanning confocal microscope. （3）Embedding test in vivo： Two longitudinal incisions were cut at the two sides of spine. The subcutaneous tissue was separated bluntly to form subcutaneous lacuna. Four pieces of PVA-collagen material were implanted in the lacuna and fixed. Nine specimens and the surrounding tissues were harvested from three rabbits each at one, four, eight and sixteen weeks postoperatively for pathological observation. MAIN

关 键 词：胶原 聚乙烯醇 生物材料 

分 类 号：R318.08[医药卫生—生物医学工程]