多种吸附剂对血浆胆红素和细胞因子吸附性能比较:新型生物材料的体外筛选实验[英文]  

Adsorptive property of novel chitosan microspheres for plasma bilirubin and cytokines: A preliminary screening in vitro of novel biomaterials

作　　者：曲娟[1] 李庭红[2] 张静[3] 陆伟[4] 张政朴[3] 

机构地区：[1]天津医科大学,天津市300070 [2]天津市第三中心医院,天津市300170 [3]南开大学高分子化学研究所 [4]天津市海河医院,天津市300350

出　　处：《中国组织工程研究与临床康复》2008年第1期170-174,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景：人工肝治疗重型肝炎要达到有效的治疗效果,必须具有吸附性能特异性强、吸附率高、血液相容性好等特性的吸附材料。目的：观察8种新型吸附剂对重型肝炎患者血浆中的胆红素和细胞因子的吸附性能。设计：对比观察。单位：天津医科大学研究生院,天津海河医院,天津市第三中心医院和南开大学高分子化学研究所。对象：全部血浆来自2004-11/2005-11天津市第三中心医院住院的重型肝炎患者30例。患者对治疗和实验均知情同意且该实验经医院伦理委员会批准。将上述患者随机分为2组：实验1组10例和实验2组20例。两组总胆红素水平分别为（377.3±147.5）,（327.6±140.1）μmol/L。方法：①吸附剂：实验选用的壳聚糖由山东青岛利中壳聚糖厂生产,相对分子质量97000,脱乙酰度85%。1#~3#吸附剂为以质量浓度1%,3%,5%相对分子量为600的聚乙二醇为致孔剂制备的壳聚糖,4#为胺基化壳聚糖,5#为苯乙烯/二乙烯苯聚合物,6#为后交连苯乙烯/二乙烯苯聚合物,7#为壳聚糖-苯乙烯/二乙烯苯聚合物,8#为壳聚糖-后交连苯乙烯/二乙烯苯聚合物。②指标检测：第一步：收集实验1组重型肝炎患者的血浆各3mL,以8种不同的吸附剂各1mL进行吸附。采用钒酸盐氧化法测定吸附前后血浆中总胆红素、直接胆红素、间接胆红素浓度,计算血浆吸附率,筛选出两种吸附率较高的吸附剂。第二步：用以上筛选出吸附率较好的两种吸附剂各1mL对实验组患者血浆（各3mL）进行胆红素和白细胞介素6、肿瘤坏死因子α的吸附实验,后两者的测定采用ELISA法。主要观察指标：吸附前后血浆中胆红素的浓度和细胞因子水平。结果：①第一步实验显示4#和5#吸附剂吸附后患者血浆总胆红素、直接胆红素、间接胆红素浓度明显降低,差异有显著性意义（P〈0.01）;其余6种吸附剂吸附前后比较,差异无显著性意义BACKGROUND： The effective therapy of artificial liver for severe hepatitis needs an absorbing material which possesses strong adsorptive property, high adsorption rate and good blood compatibility. OBJECTIVE： To study the adsorptive property of eight novel adsorbents for plasma bilirubin and cytokines in severe hepatitis patients. DESIGN： A controlled observation. SETTINGS： Graduate School of Tianjin Medical University, Tianjin Haihe Hospital, Tianjin Third Central Hospital and the Institute of Polymer Chemistry in Nankai University. PARTICIPANTS： All plasma was collected from 30 severe hepatitis patients hospitalized in Tianjin Third Central Hospital from November 2004 to November 2005. Informed consent was obtained from each patients. This experiment was approved by the hospital ethical committee. All the patients were divided into two groups at random： group l （n=10） and group 2 （n=20）. The level of total bilirubin （TBiL） before therapy in two groups was （377.3±147.5） μmol/L and （327.6± 140.1） μmol/L, respectively. METHODS： （1）Adsorbents： Chitosan （Qingdao Lizhong Chitosan Factory, Shandong） with relative molecular weight 97 000 and de-acetyl grade 85%; Adsorbents No.1-3 were prepared by using 1%, 3%, 5% polyethyleneglycol （relative molecular weight 600） as porogenic agent. Adsorbent No.4 was aminated crosslinked chitosan microspheres; Adsorbent No.5 was divinyl-benzene crosslinked macroporous polystyrene microspheres; Adsorbent No.6 was post-crosslinked macroporous divinyl-benzene styrene copolymer microspheres; Adsorbents No.7 and 8 were chitosan wrapped adsorbent No.5 and 6.（2） Detection： Step 1：3 mL plasma collected from each severe hepatitis patient in group 1 was absorbed with 1 mL of 8 kinds of adsorbents. Levels of plasma TBiL, direct bilirubin （DBiL） and indirect bilirubin （IBiL） before and after adsorption were determined by using the vanadate oxidation method to analyze the average adsorption capacity so as to screen the adsorbents

关 键 词：壳聚糖 重型肝炎 胆红素 细胞因子 

分 类 号：R318.08[医药卫生—生物医学工程]