针刀松解法对第三腰椎横突综合征大鼠下丘脑与脊髓P物质、八肽胆囊收缩素含量的影响  

Effect of Acupotomy Lysis on SP and CCK-8 Contents in Hypothalamus and Spinal Cord in Rats with Transverse Process Syndrome of the Third Lumbar Vertebra

作　　者：胡波[1] 刘琳[1] 郭长青[1] 李晓泓[1] 孙红梅[1] 刘乃刚[1] 卢婧[1] 谷世喆[1] 马惠芳[1] 

机构地区：[1]北京中医药大学针灸学院,北京100029

出　　处：《针刺研究》2008年第1期22-25,共4页Acupuncture Research

基　　金：国家重点基础研究发展计划"973"项目(2005CB523307)

摘　　要：目的:探讨针刀松解法治疗第三腰椎横突综合征的中枢镇痛机制。方法:选用SD雄性大鼠28只,随机分为4组:正常组、模型组、电针组和针刀组,每组7只。后3组首先采用一侧第三腰椎横突尖部埋置明胶海绵的方法建立第三腰椎横突综合征的动物模型,电针组和针刀组分别给予电针左侧"肾俞""腰阳关"和针刀松解干预治疗。造模28 d后取大鼠下丘脑和脊髓腰段,以酶联免疫方法检测大鼠下丘脑和脊髓腰段P物质(SP)、八肽胆囊收缩素(CCK-8)的含量。结果:模型组大鼠下丘脑与脊髓组织中SP、CCK-8的含量与正常组比较均明显升高(P<0.01);经针刀松解治疗后,SP、CCK-8的含量均较模型组降低(P<0.01);电针组下丘脑和脊髓中SP含量明显降低(P<0.05),下丘脑CCK-8的含量亦明显降低(P<0.01);针刀组下丘脑和脊髓组织中SP、CCK-8的含量更接近正常组。结论:针刀松解法对第三腰椎横突综合征大鼠中枢SP、CCK-8合成释放具有良性调节作用,针刀松解治疗的方法略优于电针。Objective To observe changes of SP and CCK-8 contents in the hypothalamus and spinal cord after performing acupotomy lysis so as to study its central analgesic effect in rats with transverse-process syndrome of the third lumbar vertebra. Methods Twenty-eight SD rats were randomly divided into normal control group, model group, acupotomy lysis group and electroacupuncture （EA） group, with 7 cases in each one. The model of the third lumbar vertebra transverse process syndrome was established by inserting a piece of gelatin sponge into the deep subfascial part of the 3rd lumbar transverse process. Acupotomy tysis was conducted 1 time/week for 2 weeks. EA （2/100 Hz, 1-2 mA） was applied to the left ＂Shenshu＂（BL 23）- ＂Yaoyangguan＂（GV 3） for 20 min/time, 3 times/week and for 2 weeks. SP and CCK-8 contents in the hypothalamus and spinal cord were detected with enzyme linked immunosorbent assay （ELISA）. Results In model group, SP and CCK-8 contents in the hypothalamus and spinal cord were increased significantly in comparison with normal control group （P〈0.01）. Compared with model group, both SP and CCK-8 contents in hypothalamus and SP content in spinal cord in acupotomy lysis and EA groups were apparently lower （P〈0.01,0.05）. CCK-8 content in spinal cord in cupotomy lysis group was lower markedly than that of model group （P〈0.01）, while no significant difference was found between model group and EA group in CCK-8 level in spinal cord （P〉0.05）. Conclusion Acupotomy iysis can downreguiate SP and CCK-8 contents in hypothalamus and spinal cord, which may contribute to its effect in relieving pain from transverse process syndrome of the third lumbar vertebra.

关 键 词：第三腰椎横突综合征 电针 针刀松解法 P物质 八肽胆囊收缩素 脊髓 下丘脑 

分 类 号：R245.319[医药卫生—针灸推拿学]