5种虫生拟青霉高产VE菌株的筛选及优化  

Screening and Optimization of High-yield Strain of VE from Five Entomogenous Paecilomyces

作　　者：张延威[1] 韩燕峰[2] 梁建东[2] 梁宗琦[2] 周礼红[2] 

机构地区：[1]贵州教育学院化学系,贵州贵阳550003 [2]贵州大学生命科学学院真菌资源研究所,贵州贵阳550025

出　　处：《安徽农业科学》2008年第4期1314-1315,1591,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目重大子项目(30499340);贵州省国际科技合作重点项目计划;贵州教育学院校基金(社科2006)

摘　　要：[目的]为从真菌中开发利用天然VE提供一定的科学依据和参考。[方法]用紫外分光光度法测定5种虫生拟青霉中天然VE的含量,采用正交试验对细脚拟青霉进行高产VE培养基的优化。[结果]细脚拟青霉中的天然VE含量最高;所得优化的培养基碳源为甘露糖,氮源为尿素,微量元素为铁,pH值自然。[结论]该研究中的真菌资源具有开发脂溶性物质的极大潜力。[ Objective ] The aim of the study was to supply some scientific basis and reference for exploiting and utilizing natural VE from fungi. [ Method] The natural VE contents in 5 entomogenous Paecilomyces were determined by ultraviolet spectrophotometry, The orthogonal experiment was used to optimize the medium with high yield of VE for Paecilomyces tenuity. [Result] The natural VE content in P, tenuipes was highest. In the optimized medium, the carbon source was mannose, the nitrogen source was ttrea, the microelement was iron and the pH value was natural. [Conclusion] The fungi resource in the research had gear potential of exploiting liposoluble matter.

关 键 词：虫草无性型 虫生真菌 代谢产物 开发应用 

分 类 号：S182[农业科学—农业基础科学]