单色光对鸡视网膜节细胞密度和大小的影响  

EFFECT OF MONOCHROMATIC LIGHT ON CELL DENSITY AND CELL SIZE OF RETINAL GANGLION CELLS IN CHICKS

作　　者：曹静[1] 王子旭[1] 陈耀星[1] 

机构地区：[1]中国农业大学动物医学院,北京100094

出　　处：《解剖学报》2008年第1期18-22,共5页Acta Anatomica Sinica

基　　金：北京市自然科学基金资助项目(6032014);高等学校博士科学点专项科研基金(2004019002);新世纪优秀人才支持计划(NCET-04-0126)

摘　　要：目的研究单色光对鸡视网膜节细胞（RGCs）分布的影响。方法采用二极管光（light-emittingdiodes,LED）灯作为光源,将60只刚出壳的雄性AA肉鸡分别饲养在红（660nm）、绿（560nm）、蓝（480nm）和白光（400--760nm）照下49d（n=15）,光照强度15 lux,光照制度23h∶1h（L∶D）。取两侧视网膜分别做Nissl染色和DiI标记RGCs,利用图像分析法观察视网膜面积、RGCs细胞密度与大小的变化。结果蓝、绿光组视网膜面积、RGCs细胞总数显著高于红、白光组（10.35%和17.07%,P〈0.05）;绿光组RGCs平均细胞密度比其他光组高14.61%（P〈0.05）;绿光组视网膜中央区（CA）的RGCs密度最高,显著高于蓝光组28.91%（P〈0.05）,其CA/NP的比值比其他光组高29.71%（P〈0.05）;蓝光组CA的RGCs胞体面积最小,颞侧周边区（TP）和鼻侧周边区（NP）的胞体最大,其TP/CA的比值比其他光组高26.65%（P〈0.05）且与其他光组相比差异显著（P〈0.05）。结论蓝、绿光可使视网膜面积、RGCs细胞总数增加;从视网膜的中央区到周边部,绿光组的RGCs密度梯度下降幅度和蓝光组的RGCs大小梯度增大幅度最明显。Objective To study the effect of monochromatic light on the distribution of retinal ganglion cells in chicks. Methods Sixty One-day-old male broilers were reared under four light treatments, red （660nm）, green （560nm）, blue （480nm） and white （400-760nm） by using LED （light-emitting diodes） as light sources until the 49th day （n=15）. Light intensity was 15 lux at the height of birds＇ heads and scheduled for 23hours of light and lhour of darkness during the entire experiment. The retinas were stained by Nissl-staining and DiI-labeling. The retinal area, cell size and density in ganglion cell layer were estimated by image analysis. Results The retinal area and RGCs＇ number in the blue and green light groups were higher than that in the red and white light groups （ 10.35% and 17.07%, P 〈 0.05）. The average cell density of RGCs in the green light group was higher than that in other groups （ 14.61%, P〈0.05）. The cell density of RGCs in the green light group was the highest at retinal central area and was higher than that in the blue light group （28.91%, P〈0.05）. The ratio of CA/NP in the green light group was higher than that in the others （29.71%, P〈 0.05）. The cell size of RGCs in the blue light group was the smallest in central area and the highest in periphery （TP and NP）. The ratio of TP/CA in the blue light group was higher than that in the others （26.65%, P〈0.05） and there was a significant difference between the others （P〈0.05）. Conclusion Blue and green lights increased retinal area and RGCs＇ number. The decreased amplitude of RGCs＇ density gradient in the green light group and the increased amplitude of RGCs＇ size gradient in the blue group were obvious from central area to peripheral area of retina.

关 键 词：单色光 视网膜节细胞 细胞密度 细胞大小 Nissl染色 DiI标记 鸡 

分 类 号：S954.52[农业科学—水产养殖]