人脐血非造血干细胞免疫原性的实验研究  

THE STUDY OF IMMUNOGENICITY OF NON-HEMATOPOIETIC STEM CELLS FROM UMBILICAL CORD BLOOD

作　　者：季凤清[1] 王屹[2] 孙海梅[1] 王丹妮[1] 曾晓蓓[1] 赵春礼[2] 杨慧[2] 

机构地区：[1]首都医科大学组织学胚胎学教研室 [2]首都医科大学北京神经科学研究所,北京100069

出　　处：《解剖学报》2008年第1期55-59,共5页Acta Anatomica Sinica

基　　金：国家重点基础研究发展计划(973)项目(2006CB500706);北京市教委科技发展计划资助项目(KM200610025005)

摘　　要：目的探讨人脐血非造血干细胞免疫原性,为脐血非造血干细胞的应用提供依据。方法通过流式细胞术检测人脐血非造血干细胞的免疫表型;体外混合淋巴细胞培养观察人脐血非造血干细胞原代、P1代、诱导分化细胞及干扰素-γ(IFN-γ)刺激异种T淋巴细胞增殖活化作用。结果人脐血非造血干细胞表达HLA-ABC,微弱表达HLA-DR,经IFN-γ处理后,未明显改变HLA-ABC、HLA-DR的表达水平。混合淋巴细胞培养未见各处理组人脐血非造血干细胞明显刺激T淋巴细胞的增殖。结论人脐血非造血干细胞无论原代、传代细胞、分化细胞及IFN-γ处理的细胞免疫原性均较弱。Objective To explore the immunogenicity of non-hematopoietic stem cells from human umbilical cord blood （HUCB）. Methods We detected HLA-ABC, HLA-DR of HUCB-nHSCs and HUCB-nHSCs treated of IFN-γ for 3 days by FACS. Then CB-nHSCs, passage 1 CB-nHSCs, differentiated CB-nHSCs, CB-nHSCs treated with IFN-γ, human neuroblastoma cell line SH-SY5Y were co-cultivated respectively with spleen lymphocytes to observe proliferation of these lymphocytes. Results The HUCB-nHSCs were positive for HLA-ABC, while few cells were positive for HLA-DR. The treatment of IFN-γ didn＇t increase the expressions of HLA-ABC and HLA-DR. In mixed lymphocyte culture, they all failed to stimulate proliferation of lymphocytes except for the SH-SY5Y group. Conclusion The non-hematopoietic stem cells from human umbilical cord blood were weak in immunogenicity.

关 键 词：人脐带血 非造血干细胞 免疫原性 脾淋巴细胞 共培养 大鼠 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]