白细胞介素-18在大鼠胚泡植入前后子宫的分布及表达  

THE DISTRIBUTION AND EXPRESSION OF IL-18 IN RAT UTERUS DURING PERI-IMPLANTATION

作　　者：张艳[1] 李文献 陈树林[1] 乔海莲[1] 杨永倩[1] 范光丽[1] 

机构地区：[1]西北农林科技大学动物医学院,杨凌712100 [2]河南省乳业研究中心,郑州450008

出　　处：《解剖学报》2008年第1期98-101,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(39670550);西北农林科技大学研究生科技创新基金(05ych020)

摘　　要：目的系统研究了白细胞介素-18(IL-18)在大鼠胚泡植入前后子宫中的分布与表达,以探讨IL-18在胚泡植入过程中可能的生理作用。方法免疫组织化学SP法,图像分析法。结果IL-18在胚泡植入前后子宫均有表达,其主要分布于子宫内膜或蜕膜的腔上皮细胞、腺上皮细胞及子宫基质细胞或蜕膜细胞,子宫肌层及部分血管内皮细胞也有微弱表达。与非妊娠大鼠相比,妊娠大鼠子宫IL-18的表达明显增加(P<0.05)。随着妊娠的进行,子宫IL-18的表达逐渐递增,并且IL-18在胚泡植入后期的表达显著高于植入前期(P<0.05)。结论IL-18可能参与胚泡植入,与妊娠的建立和维持有关,在妊娠中起重要作用。Objective To study the distribution and expression of interleukin-18 （IL-18） in rat uterus during peri-implantation and understand possible physiological regulatory roles of endogenous IL-18 in embryo implantation. Methods Immunohistochemical technique and image analysis were used. Results IL-18 protein could be detected in all the rat uteri. The positive cells were mainly distributed in the uterine endometrial or decidual luminal epithelium, the glandular epithelium, and the stroma or decidual cells; a weak expression was also found in the myometrium and some endothelial cells. Compared with the estrus group, the expression of IL-18 in pregnant rats distinctly increased （P〈0.05）, and with the developing of pregnancy, the level of IL-18 in pregnant rats was gradually enhanced. Moreover, the expression of IL-18 during post-implantation period was markedly higher than that during pre-implantation period （P〈0.05）. Conclusion IL-18 may play a role in pregnancy and regulate embryo implantation. It may be related with the establishment and maintanence of pregnancy.

关 键 词：白细胞介素-18 子宫 胚泡 植入 免疫组织化学 大鼠 

分 类 号：R321.3[医药卫生—人体解剖和组织胚胎学]