胎儿巩膜碱性成纤维细胞生长因子和转化生长因子β表达的研究  

THE STUDY ON THE EXPRESSION OF BASIC FIBROBLAST GROWTH FACTOR AND TRANSFORMING GROWTH FACTOR-BETA IN THE SCLERA OF HUMAN FETUS

作　　者：肖瑛[1] 潘庆敏[1] 高志娟[1] 孙洪义[1] 周亚滨[2] 唐伟[2] 

机构地区：[1]山东大学第二医院眼科,济南250033 [2]山东大学病原微生物研究所,济南250012

出　　处：《解剖学报》2008年第1期124-126,共3页Acta Anatomica Sinica

基　　金：山东省科技攻关计划资助项目(2005GG442027)

摘　　要：目的观察不同胎龄胎儿眼球巩膜碱性成纤维细胞生长因子（bFGF）和转化生长因子β（TGF-β）的表达情况,了解bFGF和TGF-β在正常眼球发育过程中的作用及变化规律。方法收集12-20周米非司酮配伍米索前列醇或利凡诺羊膜腔注射引产的胎儿20例,其40只眼,立即摘除眼球,取4-5mm范围巩膜,用实时定量PCR方法检测巩膜bFGF和TGF-β的表达情况。结果各胎龄阶段巩膜bFGF均有表达,胎龄12-14周时表达最高,以后逐渐下降并保持稳定水平,各胎龄阶段巩膜均有TGF-β表达,胎龄12周时表达较低,以后逐渐升高并保持稳定水平。结论各胎龄阶段胎儿巩膜均有bFGF和TGF-β的表达,bFGF和TGF-β的表达水平随着胎龄的变化而变化。Objective To evaluate the existence of basic fibroblast growth factor（bFGF） and transforming growth factor-beta（TGF-β） in the sclera in different fetal ages by real-time polymerase chain reaction （PCR）. Methods Forty human fetal eyes ranging in age from 12 to 20 pregnant weeks were carefully extracted. The sclera was carefully dissected. The expressions of bFGFand TGF-β were determined by real-time PCR, and the results were analyzed comparatively. Results bFGF was expressed in the highest amount at the 12th to 14th pregnant week and decreased thereafter, while the expression of TGF-β lowered at the 12th week and increased gradually. Conclusion bFGF and TGF-β express in the sclera of the human fetal eyes during the whole period of the fetal development and changed with fetal ages.

关 键 词：碱性成纤维细胞生长因子 转化生长因子Β 后巩膜 实时定量PCR 胎儿 

分 类 号：R321.5[医药卫生—人体解剖和组织胚胎学]