诺帝对血管内皮生长因子诱导的人脐血源性内皮祖细胞功能的影响  

Effect of Nordy on the function of endothelial progenitor cells from human umbilical cord blood induced by vascular endothelial growth factor

作　　者：张华蓉[1] 徐承平[1] 陈飞兰[1] 卞修武[1] 

机构地区：[1]第三军医大学西南医院病理学研究所,重庆400038

出　　处：《药学学报》2008年第2期133-137,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30370552)

摘　　要：探讨手性化合物诺帝（Nordy）对血管内皮生长因子（VEGF）诱导的人脐血源性内皮祖细胞（EPCs）功能的影响及其意义。应用密度梯度离心法分离新鲜人脐血的单个核细胞,接种于EGM-2培养液中培养7-10d获得内皮祖细胞（EPCs）。分别采用MTT法、Millicell-PCF培养小室系统和Matrigel内小管形成试验检测诺帝对VEGF刺激下EPCs增殖活性、迁移能力和体外形成小管样结构能力的影响。结果表明,100μmol·L^-1诺帝作用24h明显抑制EPCs增殖活性（P〈0.05）,诺帝（25-50μmol·L^-1）作用48-72h也明显抑制EPCs增殖活性（P〈0.05）。诺帝（25-100μmol·L^-1）显著抑制VEGF诱导的EPCs迁移活性和体外形成小管样结构的能力（P〈0.05）。诺帝能抑制体外VEGF诱导的人脐血源性EPCs增殖、迁移和体外小管形成能力,提示其具有抗EPCs效应。This study is to investigate whether the synthesized chiral compound Nordy has influence on the function of endothelial progenitor cells （EPCs） from human umbilical cord blood induced by vascular endothelial growth factor （VEGF）. EPCs were isolated from human umbilical cord blood by density gradient centrifugation. After cultured for 7-10 days, EPCs were prepared for detecting effect of Nordy on proliferation, migration and tubule-forming activity in Matrigel induced by VEGF. Incubation of EPCs with 100 μmol·L^ -1 Nordy for 24 h initially inhibited the proliferative capacity of EPCs induced by VEGF （P〈0.05）. Moreover, 25-50 μmol·L^ -1 Nordy also exhibited inhibitory effect at 48-72 h. In addition, 25-100 μmol·L^ -1 Nordy impaired EPCs migratory and tubule-forming capacity in vitro （P〈0.05）. Nordy could inhibit in EPCs the functions of proliferation, migration and tubulogenesis induced by VEGF in vitro, which might be a possible mechanism of its anti-EPCs effects.

关 键 词：内皮祖细胞 血管内皮生长因子 诺帝 

分 类 号：R963[医药卫生—微生物与生化药学]