光学方法分析GABA及GABAA受体在脑干听觉传入通路的作用  

THE EFFECTS OF GABAERGIC NEUROTRANSMITTERS AND GABAA RECEPTORS ON THE AUDITORY AFFERENT PATHWAY IN THE BRAINSTEM ANALYZED BY OPTICAL RECORDING

作　　者：蔡竖平[1] 房征宇[1] 杨仕明[2] 土井直 

机构地区：[1]解放军总医院老年医学研究所 [2]解放军总医院耳鼻喉头颈外科,北京100853 [3]关西医科大学耳鼻喉科,日本

出　　处：《中国应用生理学杂志》2008年第1期42-45,共4页Chinese Journal of Applied Physiology

摘　　要：目的：旨在探讨脑干听觉传入通路中GABA能神经递质及GABAA受体对电刺激位听神经传入冲动的影响。方法：使用出生后0-5 d的ddy/ddy小鼠制备脑干切片。脑片经电压敏感染料NK3041染色,电刺激与脑片相连的位听神经残端。使用16×16像素的硅光电二极管阵列测量光学信号。所采集的数据使用ARGUS-50/PDA软件分析。结果：多部位的光学记录方法显示了从位听神经到耳蜗核和前庭核的兴奋性传导的时间-空间分布。其中每一个光学成分由快峰电位样反应和慢反应组成。抑制性神经递质GABA可降低诱发的光学信号的快反应和慢反应,GABAA受体拮抗剂荷包牡丹碱可增强这些反应。结论：16×16像素的硅光电二极管阵列可记录位听神经刺激诱发的多部位光学信号,每一个光学信号含有突触前及突触后电位成分。抑制性神经递质GABA和GABAA受体拮抗剂可调节光学信号的兴奋性传导。Aim： To explore the influence of GABAergic neurotransrnitters and GABAA receptors on the auditory afferent impulses recorded in the brainstem evoked by electro-stimulation. Methods： Brainstem slices were prepared using ddy/ddy mice of postnatal 0-5th days. The brainstem slices were stained with a voltage-sensitive dye（NK3041）. The cut end of the vestibulocochlear nerve （nVIIIth） connected with slices was stimulated by a tungsten electrode, a 16 × 16 pixels silicon photodiode array apparatus was used to record the optical mapping from auditory brainstem slices. The data were analyzed by ARGUS-50/PDA software. Results： The spatial-temporal patterns of the excitatory propagation from the vestibulocochlear nerve （nVIIIth） to cochlear nucleus and vestibular nucleus were displayed with multiple-sites optical recording. The optical signal coming from one pixel consisted of a fast spike-like response and a following slow response. Inhibitory neurotransrnitter GABA decreased the fast spike-like response and following slow response of evoked optical signals, while an antagonist BMI against GABAA receptors increased the both responses. Conclusion： A 16× 16 pixel silicon photodiode array apparatus can be used to record multiple-sites optical mapping evoked by electro-stimulation to the cut end of the vestibulocochlear nerve. The every optical signal consists of both presynaptic and postsynaptic elemennts. Inhibitory neurotransrnitter GABA and an antagonist BMI of GABAA receptors can modulate the excitatory propagation of evoked optical signals.

关 键 词：光学记录 GABA GABAA受体 脑干切片 

分 类 号：R764.4[医药卫生—耳鼻咽喉科]