阿托伐他汀对自发性高血压大鼠p27蛋白表达及心肌细胞凋亡的影响  

EFFECTS OF ATORVASTATIN ON P27 PROTEIN EXPRESSION AND CARDIOMYOCYTES APOPTOSIS IN SPONTANEOUSLY HYPERTENSIVE RATS

作　　者：康兰[1] 胡申江[1] 

机构地区：[1]浙江大学医学院附属第一医院心内科,浙江杭州310003

出　　处：《中国应用生理学杂志》2008年第1期46-49,I0003,共5页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(30470715)

摘　　要：目的:观察阿托伐他汀对自发性高血压大鼠(SHR)p27蛋白表达及心肌细胞凋亡的影响。方法:将12只8周龄的雄性SHR随机分为2组(n=6);蒸馏水饲养组(DW组)与阿托伐他汀治疗组(ATV组),并以6只同周龄的正常血压大鼠(WKY)作为对照(WKY组)。分别采用RT-PCR与Western blot法检测p27的mRNA及蛋白表达水平;TUNEL法检测心肌细胞的凋亡;同时计算左室重与体重比,检测血脂水平。结果:阿托伐他汀治疗10周后,ATV组与DW组相比较,其①血脂水平及左室重、左室重与体重比均明显降低(P<0.01);②p27的mRNA及蛋白表达水平明显上调(P<0.01);③心肌细胞的凋亡率显著增高(P<0.01)。结论:阿托伐他汀能够上调SHR的p27蛋白表达水平、促进其心肌细胞的凋亡,在某种程度上可能与其改善SHR心室肥厚的效应有关。Aim： To explore the effects of atorvastatin on p27 protein expression and cardiomyocytes apoptosis in spontaneously hypertensive rats（SHR）. Methods： 12 eight-week-old SHR were randomized into 2 groups（ n = 6） ： distilled water group（DW group） and atorvastatin treated group（ATV group）. The age-matched wistar-kyoto rats（WKY）were used as controls（WKY group）. RT-PCR and Western blot were used to detect, respectively, p27 mRNA and protein expression levels. TUNEL technique was used to detect the apoptotic rate of cardiomyocytes. Meanwhile, left ventricular weight and body weight ratio（LVW/BW）, and serum lipids levels were examined in this study. Results： After 10 weeks treatment with atorvastatin, （1） serum lipids levels and LVW and LVW/BW ratio in ATV group were markedly decreased compared to DW group; （2）the apoptotic rate of cardiomyocytes in ATV group was much higher than that in DW group;（3）the rnRNA and protein levels of p27 expression in ATV group were also increased markedly compared to DW group. Conclusion： Atorvastatin upregulated the expression of p27 at its rnRNA and protein level, and also, facilitated cardiomyocytes apoptosis,which, to a certain extent, might be associated with its effect on the regulation of ventricular hypertrophy.

关 键 词：阿托伐他汀 凋亡 P27 心室肥厚 自发性高血压大鼠 

分 类 号：R541.3[医药卫生—心血管疾病]