模拟高原低氧对小鼠红细胞介电特性的影响  

EFFECTS OF SIMULATED HYPOXIA ON DIELECTRIC PROPERTIES OF MOUSE ERYTHROCYTES

作　　者：马青[1] 汤治元[1] 王钦文[1] 赵鑫[1] 

机构地区：[1]宁波大学医学院生理学与药理学研究所,浙江宁波315211

出　　处：《中国应用生理学杂志》2008年第1期90-94,共5页Chinese Journal of Applied Physiology

基　　金：浙江省分析测试科技项目(2007F70042);宁波大学王宽诚幸福基金和人才基金资助(2005030)

摘　　要：目的:探讨模拟高原低氧对小鼠红细胞介电特性的影响。方法:实验动物分成平原对照组和模拟高原低氧组,采用Agilent 4294A阻抗分析仪测量小鼠红细胞交流阻抗,通过细胞介电谱、Cole-Cole图、介电损耗因子频谱、损耗角正切频谱以及Cole-Cole方程的曲线拟合分析,观察模拟高原低氧对红细胞介电特性影响。结果:小鼠模拟海拔5000 m低氧4周后,模拟高原组的低频介电常数εl和介电增量Δε较平原对照组分别增加57%和59%;模拟高原组的低频电导率κl和高频电导率κh较平原对照组分别降低49%和11%。结论:模拟高原低氧可以引起小鼠红细胞介电性增加和导电性降低。Aim： To explore the influence of simulated altitude hypoxia on dielectric properties of mouse erythrocytes. Methods： Experimental animals were divided into the plain control group（control） and simulated altitude hypoxia group（altitude）. The AC impedance of mouse erythrocytes was measured with the Agilent 4294A impedance analyzer, the influence of simulated altitude hypoxia on dielectric properties of mouse erythrocytes was observed by cell dielectric spectroscopy, Cole-Cole plots, loss factor spectrum, loss tangent spectrum, and curve fitting analysis of Cole-Cole equation. Results： After mice were exposed to hypoxia at simulated 5000 m altitude for 4 weeks, pennittivity at low frequency （ε1） and dielectric increment（△ε） increased 57% and 59% than that of control group respectively, conductivity at low frequency（1q） and conductivity at high frequency（1q1） reduced 49 % and 11% than that of contml group respectively. Conclusion： The simulated altitude hypoxia could arise to increase dielectric capability and depress conductive performance on mouse erythrocytes.

关 键 词：模拟高原低氧 红细胞 介电特性 介电常数 电导率 

分 类 号：R852.11[医药卫生—航空、航天与航海医学] R331.1[医药卫生—临床医学]